ArticlePlant reproduction2019
Transcriptomics of Arabidopsis sperm cells at single-cell resolution.
Article in Plant reproduction, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
12 citing papers in PubMed.
- Bioinformatics insights into plant genomic imprinting: approaches, challenges, and future perspectives.Briefings in functional genomics · 2026Review
- A single-cell, spatial transcriptomic atlas of the Arabidopsis life cycle.Nature plants · 2025Article
- Bioimaging insights into structural pathways of cell-to-cell communication within the male (MGU) and female (FGU) germ units of Arabidopsis thaliana.Plant cell reports · 2025Article
- Analysis of Xylem Cells by Nucleus-Based Transcriptomics and Chromatin Profiling.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Fifty years of sperm cell isolations: from structural to omic studies.Journal of experimental botany · 2023Article
- Cell-type-specific alternative splicing in the Arabidopsis germline.Plant physiology · 2023Article
- Distinct chromatin signatures in the Arabidopsis male gametophyte.Nature genetics · 2023Article
- Opportunities and challenges in the application of single-cell and spatial transcriptomics in plants.Frontiers in plant science · 2023Review
- The egg cell is preferentially fertilized in Arabidopsis double fertilization.Journal of integrative plant biology · 2022Article
- High-throughput single-cell transcriptomics reveals the female germline differentiation trajectory in Arabidopsis thaliana.Communications biology · 2021Article
- Plant single-cell solutions for energy and the environment.Communications biology · 2021Review
- Enhancing Our Understanding of Plant Cell-to-Cell Interactions Using Single-Cell Omics.Frontiers in plant science · 2021Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
key messageWe present a detailed protocol for isolation of single sperm cells and transcriptome analysis to study variation in gene expression between sperm cells. Male gametophyte development in flowering plants begins with a microspore mother cell, which upon two consecutive cell divisions forms a mature pollen grain containing a vegetative nucleus and two sperm cells. Pollen development is a highly dynamic process, involving changes at both the transcriptome and epigenome levels of vegetative nuclei and the pair of sperm cells that have their own cytoplasm and nucleus. While the overall transcriptome of Arabidopsis pollen development is well documented, studies at single-cell level, in particular of sperm cells, are still lacking. Such studies would be essential to understand whether and how the two sperm cells are transcriptionally different, in particular once the pollen tube grows through the transmitting tissue of the pistil. Here we describe a detailed protocol for isolation of single sperm cells from growing pollen tubes and analysis of their transcriptome.
Indexed as
Identifiers
30675644What Socratic holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.