ArticleToxins2019
Aptamer-Based Fluorometric Ochratoxin A Assay Based on Photoinduced Electron Transfer.
Article in Toxins, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
8 citing papers in PubMed, 34 citations in OpenAlex.
- A Generalizable Screening Platform for Developing Functional Aptasensors.Analytical chemistry · 2025Article
- Detection of ochratoxin A using a "turn-on" fluorescence assay based on guanine quenching of the aptamer.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2023Article
- Aptamer Molecular Beacon Sensor for Rapid and Sensitive Detection of Ochratoxin A.Molecules (Basel, Switzerland) · 2022Article
- A Label-Free Fluorescence Aptasensor Based on G-Quadruplex/Thioflavin T Complex for the Detection of Trypsin.Molecules (Basel, Switzerland) · 2022Article
- Development of Fluorescent Aptasensors Based on G-Quadruplex Quenching Ability for Ochratoxin A and Potassium Ions Detection.Biosensors · 2022Article
- Design of a Quencher-Free Fluorescent Aptasensor for Ochratoxin A Detection in Red Wine Based on the Guanine-Quenching Ability.Biosensors · 2022Article
- Amplified Fluorescent Aptasensor for Ochratoxin A Assay Based on Graphene Oxide and RecJToxins · 2020Article
- Fluorescent Method for the Detection of Biothiols Using an Ag⁺-Mediated Conformational Switch.Sensors (Basel, Switzerland) · 2019Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
This study describes a novel quencher-free fluorescent method for ochratoxin A (OTA) detection based on the photoinduced electron transfer (PIET) between guanine and fluorophore. In the absence of OTA, carboxyfluorescein (FAM)-labeled aptamer can partly hybridize with the complementary strand of OTA aptamer (OTA-cAPT), which contains four guanines at its 3'-end. As a result, the fluorescence of FAM is quenched due to PIET and stacked guanines. In the presence of OTA, FAM-labeled OTA aptamer can bind specifically to OTA, and thereby the high fluorescence intensity of the dye can be maintained. Under the optimal conditions, the method had a detection limit of 1.3 nM. In addition, the method we proposed is highly sensitive and specific for OTA. Furthermore, the method was proven to be reliable based on its successful application in the detection of OTA in red wine samples. Therefore, this promising, facile, and quencher-free method may be applied to detect other toxins by using other appropriate aptamers.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.