Evidence map›Paper›PMID 30678730›Full record

ArticleStem cell research & therapy2019

Nanosecond pulsed electric fields enhanced chondrogenic potential of mesenchymal stem cells via JNK/CREB-STAT3 signaling pathway.

Tong Ning, Jinsong Guo, Kun Zhang, Kejia Li, Jue Zhang, Zheng Yang, Zigang Ge

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stem cell research & therapy, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.

0numbers the graph read from it
0cells of the map it votes in
26citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

26 citing papers in PubMed.

  1. Article
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  3. Electrical Stimulation Therapy - Dedicated to the Perfect Plastic Repair.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025
    Review
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  12. Article
  13. Electrical Stimulation in Cartilage Tissue Engineering.Bioengineering (Basel, Switzerland) · 2023
    Review
  14. Review
  15. Review
  16. Article
  17. Article
  18. Article
  19. Article
  20. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Tong Ning, Peking-Tsinghua Center for Life Sciences, Beijing, 100871, China.
Jinsong GuoInstitute of Biomechanics and Biomedical Engineering, College of Engineering, Peking University, Beijing, 100871, China.
Kun ZhangDepartment of Biomedical Engineering, College of Engineering, Peking University, Beijing, 100871, China.
Kejia LiDepartment of Biomedical Engineering, College of Engineering, Peking University, Beijing, 100871, China.
Jue ZhangInstitute of Biomechanics and Biomedical Engineering, College of Engineering, Peking University, Beijing, 100871, China. zhangjuezjh@gmail.com.
Zheng YangTissue Engineering Program, Life Sciences Institute, National University of Singapore, 27 Medical Drive, Singapore, 117510, Singapore.
Zigang GeDepartment of Biomedical Engineering, College of Engineering, Peking University, Beijing, 100871, China. gez@pku.edu.cn.ORCID 0000-0002-5202-0973

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundNanosecond pulsed electric fields (nsPEFs) can produce more significant biological effects than traditional electric fields and have thus attracted rising attention in developing medical applications based on short pulse duration and high field strength, such as effective cancer therapy. However, little is known about their effects on the differentiation of stem cells. Furthermore, mechanisms of electric fields on chondrogenic differentiation of mesenchymal stem cells (MSCs) remain elusive, and effects of electric fields on cartilage regeneration need to be verified in vivo. Here, we aimed to study the effects of nsPEFs on chondrogenic differentiation of MSCs in vitro and in vivo and further to explore the mechanisms behind the phenomenon.

methodsThe effects of nsPEF-preconditioning on chondrogenic differentiation of mesenchymal stem cells (MSCs) in vitro were evaluated using cell viability, gene expression, glycosaminoglycan (sGAG) content, and histological staining, as well as in vivo cartilage regeneration in osteochondral defects of rats. Signaling pathways were investigated with protein expression and gene expression, respectively.

resultsnsPEF-preconditioning with proper parameters (10 ns at 20 kV/cm, 100 ns at 10 kV/cm) significantly potentiated chondrogenic differentiation capacity of MSCs with upregulated cartilaginous gene expression and increased matrix deposition through activation of C-Jun NH2-terminal kinase (JNK) and cAMP-response element binding protein (CREB), followed by activation of downstream signal transducer and activator of transcription (STAT3). Implantation of nsPEF-preconditioned MSCs significantly enhanced cartilage regeneration in vivo, compared with implantation of non-nsPEF-preconditioned MSCs.

conclusionThis study demonstrates a unique approach of nsPEF treatment to potentiate the chondrogenic ability of MSCs through activation of JNK/CREB-STAT3 that could have translational potential for MSC-based cartilage regeneration.

Indexed as

Cell DifferentiationChondrogenesisElectricityHumansMesenchymal Stem CellsSignal TransductionSTAT3 Transcription FactorSTAT3 protein, humanSTAT3 Transcription FactorCartilage regenerationChondrogenic differentiationElectrical stimulationPulsed electric fieldsStem cells

Identifiers

PMID30678730
PMCPMC6346554

What Socratic holds

Textmetadata
LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.