ArticleScientific reports2019
Molecular cloning, expression, and functional analysis of the chitin synthase 1 gene and its two alternative splicing variants in the white-backed planthopper, Sogatella furcifera (Hemiptera: Delphacidae).
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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15 citing papers in PubMed, 34 citations in OpenAlex.
- Molecular and functional analysis of phosphoacetylglucosamine mutase (PAGM) and Glucose-6-phosphate isomerase (G6PI) gene in the white-backed planthopper Sogatella furcifera (Hemiptera: Delphacidae).Scientific reports · 2026Article
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- Molecular identification and functional analysis of chitinase genes reveal their importance in the metamorphosis of Sarcophaga peregrina (Diptera: Sarcophagidae).Journal of insect science (Online) · 2023Article
- Silencing ofBiomolecules · 2023Article
- Both symbionts and environmental factors contribute to shape the microbiota in a pest insect,Frontiers in microbiology · 2023Article
- Knockdown of Two Trehalase Genes by RNA Interference Is Lethal to the White-Backed PlanthopperBiomolecules · 2022Article
- Characterization of Chitin Synthase A cDNA fromInsects · 2022Article
- Molecular Characterization of UDP-Genes · 2022Article
- Identification and RNAi-Based Functional Analysis of Four Chitin Deacetylase Genes in Sogatella furcifera (Hemiptera: Delphacidae).Journal of insect science (Online) · 2021Article
- Review
- Chitin Synthases Are Critical for Reproduction, Molting, and Digestion in the Salmon Louse (Life (Basel, Switzerland) · 2021Article
- Role of phosphoglucomutase in regulating trehalose metabolism in3 Biotech · 2020Article
- Glucose Utilization in the Regulation of Chitin Synthesis in Brown Planthopper.Journal of insect science (Online) · 2019Article
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6 authors at 3 institutions in 1 country.
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Abstract
Chitin synthase is responsible for chitin synthesis in the cuticles and cuticular linings of other tissues in insects. We cloned two alternative splicing variants of the chitin synthase 1 gene (SfCHS1) from the white-backed planthopper, Sogatella furcifera. The full-length cDNA of the two variants (SfCHS1a and SfCHS1b) consists of 6408 bp, contains a 4719-bp open reading frame encoding 1572 amino acids, and has 5' and 3' non-coding regions of 283 and 1406 bp, respectively. The two splicing variants occur at the same position in the cDNA sequence between base pairs 4115 and 4291, and consist of 177 nucleotides that encode 59 amino acids but show 74.6% identity at the amino acid level. Analysis in different developmental stages showed that expression of SfCHS1 and SfCHS1a were highest just after molting, whereas SfCHS1b reached its highest expression level 2 days after molting. Further, SfCHS1 and SfCHS1a were mainly expressed in the integument, whereas SfCHS1b was predominately expressed in the gut and fat body. RNAi-based gene silencing inhibited transcript levels of the corresponding mRNAs in S. furcifera nymphs injected with double-stranded RNA of SfCHS1, SfCHS1a, and SfCHS1b, resulted in malformed phenotypes, and killed most of the treated nymphs. Our results indicate that SfCHS1 may be a potential target gene for RNAi-based S. furcifera control.
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