Evidence map›Paper›PMID 30729433›Full record

ArticleEnvironmental science and pollution research international2019

α-Amylase and α-glucosidase inhibitor effects and pancreatic response to diabetes mellitus on Wistar rats of Ephedra alata areal part decoction with immunohistochemical analyses.

Jihene Ben Lamine, Mouhamed Ali Boujbiha, Sabra Dahane, Amal Ben Cherifa, Aida Khlifi, Hassiba Chahdoura, Mouhamed Taher Yakoubi, Salima Ferchichi, Nacer El Ayeb, Lotfi Achour

Abstract read
PubMed Publisher
In one paragraph

Article in Environmental science and pollution research international, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
3.5field-weighted citation impact, top 7% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed, 43 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 3 institutions in 1 country.

Jihene Ben LamineInstitut Supérieur de Biotechnologie de Monastir, Laboratoire de Recherche : Bioressources, Biologie Intégrative & Valorisation, Université de Monastir, LR14ES06, BP 74, 5000, Monastir, Tunisia. jihenebenlamine@gmail.com.ORCID http://orcid.org/0000-0002-2641-7707
Mouhamed Ali BoujbihaInstitut Supérieur de Biotechnologie de Monastir, Laboratoire de Recherche : Bioressources, Biologie Intégrative & Valorisation, Université de Monastir, LR14ES06, BP 74, 5000, Monastir, Tunisia.
Sabra DahaneInstitut Supérieur de Biotechnologie de Monastir, Laboratoire de Recherche : Bioressources, Biologie Intégrative & Valorisation, Université de Monastir, LR14ES06, BP 74, 5000, Monastir, Tunisia.
Amal Ben CherifaInstitut Supérieur de Biotechnologie de Monastir, Laboratoire de Recherche : Bioressources, Biologie Intégrative & Valorisation, Université de Monastir, LR14ES06, BP 74, 5000, Monastir, Tunisia.
Aida KhlifiInstitut Supérieur de Biotechnologie de Monastir, Laboratoire de Recherche : Bioressources, Biologie Intégrative & Valorisation, Université de Monastir, LR14ES06, BP 74, 5000, Monastir, Tunisia.
Hassiba ChahdouraInstitut Supérieur de Biotechnologie de Monastir, Laboratoire de Recherche : Bioressources, Biologie Intégrative & Valorisation, Université de Monastir, LR14ES06, BP 74, 5000, Monastir, Tunisia.
Mouhamed Taher YakoubiLaboratoire d'anatomie et pathologie, Centre Hôpital Universitaire Farhat Hached, Sousse, Tunisia.
Salima FerchichiLaboratoire de biochimie, Centre Hôpital Universitaire Farhat Hached, Sousse, Tunisia.
Nacer El AyebInstitut Supérieur de Biotechnologie de Monastir, Laboratoire de Recherche : Bioressources, Biologie Intégrative & Valorisation, Université de Monastir, LR14ES06, BP 74, 5000, Monastir, Tunisia.
Lotfi AchourInstitut Supérieur de Biotechnologie de Monastir, Laboratoire de Recherche : Bioressources, Biologie Intégrative & Valorisation, Université de Monastir, LR14ES06, BP 74, 5000, Monastir, Tunisia.
University of Monastir · TNHôpital Farhat Hached · TNTunis University · TN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Ephedra alata, known as a medicinal plant in China, was used in this study as aqueous extract from aerial parts, for diabetes mellitus treatment. This study was carried out on two parts, in vitro, we tested the effect of the studied extract on the inhibition of α-glucosidase and α-amylase activities, and in vivo on Wistar male rats receiving alloxan intraperitoneally at a rate of 125 mg/kg. Extract (100, 200, and 300 mg/kg of body weight) was administrated for 28 days by oral gavage. Blood glucose, amylase, lipase, and lipid profile level were determined. Oxidative stress was evaluated by enzymatic activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx), and by estimation of lipid peroxidation and protein carbonyl (PC) level. Histopathological changes in pancreas were investigated under photonic microscopy using immunohistochemical procedure. Our findings showed that aqueous extract inhibited in vitro both α-glucosidase and α-amylase activities and its use in vivo at 300 mg/kg of body weight restored pancreas weight and weight gain, ameliorated significantly (p ˂ 0.05) biochemical parameters; it prevented the increase in lipid and protein oxidation and the decrease in enzymatic and non-enzymatic defense system. Histological study of treated animals showed a comparable healed regeneration of beta cells.

Indexed as

alpha-AmylasesAnimalsBlood GlucoseCatalaseChinaDiabetes Mellitus, ExperimentalEphedraGlutathione PeroxidaseGlycoside Hydrolase InhibitorsHypoglycemic AgentsLipid PeroxidationMaleOxidative StressPancreasPlant ExtractsRatsalpha-AmylasesBlood GlucoseCatalaseGlutathione PeroxidaseGlycoside Hydrolase InhibitorsHypoglycemic AgentsPlant ExtractsSuperoxide DismutaseAlloxanDiabetes mellitusEphedra alataOxidative stressPancreas

Identifiers

PMID30729433
OpenAlexW2913558146

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.