Evidence mapPaperPMID 30736731Full record

ArticleBMC microbiology2019

Lactobacillus mucosae DPC 6426 as a bile-modifying and immunomodulatory microbe.

Paul M Ryan, Ellen H Stolte, Lis E E London, Jerry M Wells, Sarah L Long, Susan A Joyce, Cormac G M Gahan, Gerald F Fitzgerald, R Paul Ross, Noel M Caplice and 1 more

Open access · goldAbstract read
In one paragraph

Article in BMC microbiology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.

0numbers the graph read from it
0cells of the map it votes in
23citing papers in PubMed
4.8field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

23 citing papers in PubMed, 44 citations in OpenAlex.

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  17. Harnessing the potential ofFuture science OA · 2021
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 4 institutions in 2 countries.

Paul M RyanTeagasc Food Research Centre, Food Biosciences Department, Moorepark, Fermoy, Co, Cork, Ireland.
Ellen H StolteHost-Microbe Interactomics, University of Wageningen, Animal Sciences Department, Wageningen, The Netherlands.
Lis E E LondonTeagasc Food Research Centre, Food Biosciences Department, Moorepark, Fermoy, Co, Cork, Ireland.
Jerry M WellsHost-Microbe Interactomics, University of Wageningen, Animal Sciences Department, Wageningen, The Netherlands.
Sarah L LongAPC Microbiome Ireland, University College Cork, Cork, Ireland.
Susan A JoyceAPC Microbiome Ireland, University College Cork, Cork, Ireland.
Cormac G M GahanSchool of Microbiology, University College Cork, Cork, Ireland.
Gerald F FitzgeraldSchool of Microbiology, University College Cork, Cork, Ireland.
R Paul RossAPC Microbiome Ireland, University College Cork, Cork, Ireland.
Noel M CapliceCentre for Research in Vascular Biology, University College Cork, Cork, Ireland.
Catherine StantonTeagasc Food Research Centre, Food Biosciences Department, Moorepark, Fermoy, Co, Cork, Ireland. catherine.stanton@teagasc.ie.
University College Cork · IEAPC Microbiome Institute · IETeagasc - The Irish Agriculture and Food Development Authority · IEWageningen University & Research · NL

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundLactobacillus mucosae DPC 6426 has previously demonstrated potentially cardio-protective properties, in the form of dyslipidaemia and hypercholesterolemia correction in an apolipoprotein-E deficient mouse model. This study aims to characterise the manner in which this microbe may modulate host bile pool composition and immune response, in the context of cardiovascular disease. Lactobacillus mucosae DPC 6426 was assessed for bile salt hydrolase activity and specificity. The microbe was compared against several other enteric strains of the same species, as well as a confirmed bile salt hydrolase-active strain, Lactobacillus reuteri APC 2587.

resultsQuantitative bile salt hydrolase assays revealed that enzymatic extracts from Lactobacillus reuteri APC 2587 and Lactobacillus mucosae DPC 6426 demonstrate the greatest activity in vitro. Bile acid profiling of porcine and murine bile following incubation with Lactobacillus mucosae DPC 6426 confirmed a preference for hydrolysis of glyco-conjugated bile acids. In addition, the purified exopolysaccharide and secretome of Lactobacillus mucosae DPC 6426 were investigated for immunomodulatory capabilities using RAW264.7 macrophages. Gene expression data revealed that both fractions stimulated increases in interleukin-6 and interleukin-10 gene transcription in the murine macrophages, while the entire secretome was necessary to increase CD206 transcription. Moreover, the exopolysaccharide elicited a dose-dependent increase in nitric oxide and interleukin-10 production from RAW264.7 macrophages, concurrent with increased tumour necrosis factor-α secretion at all doses.

conclusionsThis study indicates that Lactobacillus mucosae DPC 6426 modulates both bile pool composition and immune system tone in a manner which may contribute significantly to the previously identified cardio-protective phenotype.

Indexed as

ImmunomodulationAmidohydrolasesAnimalsBileCardiovascular DiseasesGlycosyltransferasesHydrolysisInterleukin-10Interleukin-6LactobacillusLectins, C-TypeLimosilactobacillus reuteriMacrophagesMannose-Binding LectinsMannose ReceptorMiceAmidohydrolasescholoylglycine hydrolaseGlycosyltransferasesIL10 protein, mouseInterleukin-10Interleukin-6interleukin-6, mouseLectins, C-TypeMannose-Binding LectinsMannose ReceptorNitric OxidePolysaccharides, BacterialReceptors, Cell SurfaceTumor Necrosis Factor-alphaBile acidBile salt hydrolase (BSH)CVDExopolysaccharideHypercholesterolaemia

Identifiers

PMID30736731
PMCPMC6368806
OpenAlexW2921211734

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.