ArticleNature nanotechnology2019
DNA nanodevices map enzymatic activity in organelles.
Article in Nature nanotechnology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
30 citing papers in PubMed, 87 citations in OpenAlex.
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- FRET-based reporter assesses lysosomal DNA-degradation ability in live cells.Sensors and actuators reports · 2025Article
- Establishing Detection Methods for Okadaic Acid Aptamer-Target Interactions: Insights from Computational and Experimental Approaches.Foods (Basel, Switzerland) · 2025Article
- Functional Nucleic Acid Nanostructures for Mitochondrial Targeting: The Basis of Customized Treatment Strategies.Molecules (Basel, Switzerland) · 2025Review
- Quantitative Chemical Imaging of Organelles.Accounts of chemical research · 2024Article
- A DNA nanodevice for mapping sodium at single-organelle resolution.Nature biotechnology · 2024Article
- Bidirectional linkage of DNA barcodes for the multiplexed mapping of higher-order protein interactions in cells.Nature biomedical engineering · 2024Article
- High-fidelity imaging of a tumour-associated lysosomal enzyme with an acceptor engineering-boosted near-infrared fluorescent probe.Chemical science · 2024Article
- Review
- A mechanism of lysosomal calcium entry.Science advances · 2024Article
- Light and endogenous enzyme triggered plasmonic antennas for accurate subcellular molecular imaging with enhanced spatial resolution.Chemical science · 2024Article
- Controllable mitochondrial aggregation and fusion by a programmable DNA binder.Chemical science · 2023Article
- Using Single-Molecule FRET to Evaluate DNA Nanodevices at Work.Methods in molecular biology (Clifton, N.J.) · 2023Article
- The evolution of organellar calcium mapping technologies.Cell calcium · 2022Review
- DNA-Based Molecular Machines.JACS Au · 2022Review
- Development of the DNA-based biosensors for high performance in detection of molecular biomarkers: More rapid, sensitive, and universal.Biosensors & bioelectronics · 2022Review
- A DNA nanodevice boosts tumour immunity.Nature nanotechnology · 2021Article
- A lysosome-targeted DNA nanodevice selectively targets macrophages to attenuate tumours.Nature nanotechnology · 2021Article
- Tubular lysosomes harbor active ion gradients and poise macrophages for phagocytosis.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Modulation of Gold Nanorod Growth via the Proteolysis of Dithiol Peptides for Enzymatic Biomarker Detection.ACS applied materials & interfaces · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Cellular reporters of enzyme activity are based on either fluorescent proteins or small molecules. Such reporters provide information corresponding to wherever inside cells the enzyme is maximally active and preclude minor populations present in subcellular compartments. Here we describe a chemical imaging strategy to selectively interrogate minor, subcellular pools of enzymatic activity. This new technology confines the detection chemistry to a designated organelle, enabling imaging of enzymatic cleavage exclusively within the organelle. We have thus quantitatively mapped disulfide reduction exclusively in endosomes in Caenorhabditis elegans and identified that exchange is mediated by minor populations of the enzymes PDI-3 and TRX-1 resident in endosomes. Impeding intra-endosomal disulfide reduction by knocking down TRX-1 protects nematodes from infection by Corynebacterium diphtheriae, revealing the importance of this minor pool of endosomal TRX-1. TRX-1 also mediates endosomal disulfide reduction in human cells. A range of enzymatic cleavage reactions in organelles are amenable to analysis by this new reporter strategy.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.