ArticleScientific reports2019
Critical Functionality Effects from Storage Temperature on Human Induced Pluripotent Stem Cell-Derived Retinal Pigment Epithelium Cell Suspensions.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers, 2 of them syntheses that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
14 citing papers in PubMed, 2 syntheses or guidelines pooled it, 28 citations in OpenAlex.
- Immunosuppression in stem cell clinical trials of neural and retinal cell types: A systematic review.PloS one · 2024Pooled it
- Concise Review: Considering Optimal Temperature for Short-Term Storage of Epithelial Cells.Frontiers in medicine · 2021Pooled it
- Effects of ocular hypothermia: potential perspectives in vitreoretinal surgery.International journal of retina and vitreous · 2025Review
- Effect of the Sugar Present in the Culture Medium on the Preservation of Human RPE Cell Suspensions.Translational vision science & technology · 2025Article
- Retinal cytoarchitecture is preserved in an organotypic perfused human and porcine eye model.Acta neuropathologica communications · 2024Article
- Review
- Investigation of the effectiveness of gelatin hydrolysate in human iPS-RPE cell suspension transplantation.Regenerative therapy · 2024Article
- Self-organization, quality control, and preclinical studies of human iPSC-derived retinal sheets for tissue-transplantation therapy.Communications biology · 2023Article
- Changes in serum pigment epithelium-derived factor levels after kidney transplantation in patients with end-stage renal disease.Renal failure · 2022Article
- Determining the optimal stage for cryopreservation of human embryonic stem cell-derived retinal pigment epithelial cells.Stem cell research & therapy · 2022Article
- Human iPS cell derived RPE strips for secure delivery of graft cells at a target place with minimal surgical invasion.Scientific reports · 2021Article
- Pluripotent Stem Cells for Cell Therapy.Methods in molecular biology (Clifton, N.J.) · 2021Article
- The Effects of Prolonged Storage on ARPE-19 Cells Stored at Three Different Storage Temperatures.Molecules (Basel, Switzerland) · 2020Article
- Transient rho-associated coiled-coil containing kinase (ROCK) inhibition on human retinal pigment epithelium results in persistent Rho/ROCK downregulation.Biochemistry and biophysics reports · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Human induced pluripotent stem cell (hiPSC)-derived retinal pigment epithelium (hiPSC-RPE) cells suspension have the potential for regenerative treatment. However, practical regenerative applications with hiPSC-RPE cells require the development of simple and cost-effective non-freezing preservation methods. We investigated the effect of non-freezing temperatures on suspended hiPSC-RPE cells in various conditions and analysed mechanisms of cell death, anoikis, Rho GTPases, hypoxia, microtubule destruction, and cell metabolism. Cells stored at 37 °C had the lowest viability due to hypoxia from high cell metabolism and cell deposits, and cells preserved at 4 °C were damaged via microtubule fragility. Cell suspensions at 16 °C were optimal with drastically reduced apoptosis and negligible necrosis. Moreover, surviving cells proliferated and secreted key proteins normally, compared to cells without preservation. hiPSC-RPE cell suspensions were optimally preserved at 16 °C. Temperatures above or below the optimal temperature decreased cell viability significantly yet differentially by mechanisms of cell death, cellular metabolism, microtubule destruction, and oxygen tension, all relevant to cell conditions. Surviving cells are expected to function as grafts where high cell death is often reported. This study provides new insight into various non-freezing temperature effects on hiPSC-RPE cells that are highly relevant to clinical applications and may improve cooperation between laboratories and hospitals.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.