ArticleJournal of molecular biology2019
A High-Resolution Luminescent Assay for Rapid and Continuous Monitoring of Protein Translocation across Biological Membranes.
Article in Journal of molecular biology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
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Who cites it
30 citing papers in PubMed.
- Functional mapping and engineering of the Sec translocon unlocked by a cell-free system.Science advances · 2026Article
- Transmembrane domain switching controls PINK1 import and fate in mitochondria.The EMBO journal · 2026Article
- Mitochondrial presequences harbor variable strengths to maintain organellar function.The Journal of cell biology · 2026Article
- Whole Cell Luminescence-Based Screen for Inhibitors of the Bacterial Sec Machinery.Biochemistry · 2024Article
- High-throughput functional analysis provides novel insight into type VII secretion inOpen biology · 2024Article
- A group B streptococcal type VII secreted LXG toxin mediates interbacterial competition and colonization of the female genital tract.bioRxiv : the preprint server for biology · 2024Article
- Development of a split-luciferase assay to establish optimal protein secretion conditions for protein production byMicrobiology (Reading, England) · 2024Article
- Rescue of mitochondrial import failure by intercellular organellar transfer.Nature communications · 2024Article
- Structure of a tripartite protein complex that targets toxins to the type VII secretion system.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Dynamic coupling of fast channel gating with slow ATP-turnover underpins protein transport through the Sec translocon.The EMBO journal · 2024Article
- The MitoLuc assay for the analysis of the mechanism of mitochondrial protein import.Methods in enzymology · 2024Article
- MitoLuc: A Luminescence-Based Assay to Study Real-Time Protein Import into Mitochondria.Methods in molecular biology (Clifton, N.J.) · 2024Article
- A type VII-secreted lipase toxin with reverse domain arrangement.Nature communications · 2023Article
- A unifying mechanism for protein transport through the core bacterial Sec machinery.Open biology · 2023Review
- Aggregation-prone Tau impairs mitochondrial import, which affects organelle morphology and neuronal complexity.Journal of cell science · 2023Article
- The MitoLuc Assay System for Accurate Real-Time Monitoring of Mitochondrial Protein Import Within Mammalian Cells.Journal of molecular biology · 2023Article
- A quantitative fluorescence-based approach to study mitochondrial protein import.EMBO reports · 2023Article
- Bioluminescence Goes Dark: Boosting the Performance of Bioluminescent Sensor Proteins Using Complementation Inhibitors.ACS sensors · 2022Article
- Experimental and Analytical Framework for "Mix-and-Read" Assays Based on Split Luciferase.ACS omega · 2022Article
- Towards a molecular mechanism underlying mitochondrial protein import through the TOM and TIM23 complexes.eLife · 2022Article
Corrections and comments
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Authors and funding
9 authors.
Funding
Abstract
Protein translocation is a fundamental process in biology. Major gaps in our understanding of this process arise due the poor sensitivity, low time resolution and irreproducibility of translocation assays. To address this, we applied NanoLuc split-luciferase to produce a new strategy for measuring protein transport. The system reduces the timescale of data collection from days to minutes and allows for continuous acquisition with a time resolution in the order of seconds, yielding kinetics parameters suitable for mechanistic elucidation and mathematical fitting. To demonstrate its versatility, we implemented and validated the assay in vitro and in vivo for the bacterial Sec system and the mitochondrial protein import apparatus. Overall, this technology represents a major step forward, providing a powerful new tool for fundamental mechanistic enquiry of protein translocation and for inhibitor (drug) screening, with an intensity and rigor unattainable through classical methods.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.