ArticleScientific reports2019
Guided STED nanoscopy enables super-resolution imaging of blood stage malaria parasites.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Article
- Review
- Transmembrane proteins mediate basal complex assembly and individual daughter cell formation in malaria parasites.Science advances · 2026Article
- Imaging malaria parasites across scales and time.Journal of microscopy · 2026Review
- 3D quantitative phase imagingRSC advances · 2025Article
- Noninvasive in vivo photoacoustic detection of malaria with Cytophone in Cameroon.Nature communications · 2024Article
- OpenSTED: open-source dynamic intensity minimum system for stimulated emission depletion microscopy.Neurophotonics · 2024Article
- Nanoscale insights into hematology: super-resolved imaging on blood cell structure, function, and pathology.Journal of nanobiotechnology · 2024Review
- Elucidating the spatio-temporal dynamics of the Plasmodium falciparum basal complex.PLoS pathogens · 2024Article
- Imagining the future of optical microscopy: everything, everywhere, all at once.Communications biology · 2023Review
- Plasmodium schizogony, a chronology of the parasite's cell cycle in the blood stage.PLoS pathogens · 2023Review
- [Frontiers and development in live-cell super-resolution fluorescence microscopy].Sheng wu yi xue gong cheng xue za zhi = Journal of biomedical engineering = Shengwu yixue gongchengxue zazhi · 2023Review
- Article
- Towards rainbow portable Cytophone with laser diodes for global disease diagnostics.Scientific reports · 2022Article
- Live-cell fluorescence imaging of microgametogenesis in the human malaria parasite Plasmodium falciparum.PLoS pathogens · 2022Article
- Expansion microscopy provides new insights into the cytoskeleton of malaria parasites including the conservation of a conoid.PLoS biology · 2021Article
- Methods Used to Investigate theFrontiers in cellular and infection microbiology · 2021Review
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4 authors.
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Abstract
Malaria remains a major burden world-wide, but the disease-causing parasites from the genus Plasmodium are difficult to study in vitro. Owing to the small size of the parasites, subcellular imaging poses a major challenge and the use of super-resolution techniques has been hindered by the parasites' sensitivity to light. This is particularly apparent during the blood-stage of the Plasmodium life cycle, which presents an important target for drug research. The iron-rich food vacuole of the parasite undergoes disintegration when illuminated with high-power lasers such as those required for high resolution in Stimulated Emission Depletion (STED) microscopy. This causes major damage to the sample precluding the use of this super-resolution technique. Here we present guided STED, a novel adaptive illumination (AI) STED approach, which takes advantage of the highly-reflective nature of the iron deposit in the cell to identify the most light-sensitive parts of the sample. Specifically in these parts, the high-power STED laser is deactivated automatically to prevent local damage. Guided STED nanoscopy finally allows super-resolution imaging of the whole Plasmodium life cycle, enabling multicolour imaging of blood-stage malaria parasites with resolutions down to 35 nm without sample destruction.
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