Evidence map›Paper›PMID 30890503›Full record

ArticleNan fang yi ke da xue xue bao = Journal of Southern Medical University2019

[Overexpression of autophagy-related gene 3 promotes autophagy and inhibits salinomycin-induced apoptosis in breast cancer MCF-7 cells].

Fang Li, Guo Huang, Ping Peng, Yao Liu, Shuanghui Li, Luogen Liu, Yunsheng Zhang

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Article in Nan fang yi ke da xue xue bao = Journal of Southern Medical University, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact, top 88% of its field
1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 0 citations in OpenAlex.

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4 · The record

Corrections and comments

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5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Fang LiDepartment of Chemistry, Hunan Polytechnic of Environment and Biology, Hengyang 421005, China.
Guo HuangClinical Research Center For Breast and Thyroid Disease Prevention of Hunan Province, Hengyang 421001, China.
Ping PengClinical Research Institute, Second Affiliated Hospital, University of South China, Hengyang 421001, China.
Yao LiuClinical Research Institute, Second Affiliated Hospital, University of South China, Hengyang 421001, China.
Shuanghui LiClinical Research Institute, Second Affiliated Hospital, University of South China, Hengyang 421001, China.
Luogen LiuClinical Research Institute, Second Affiliated Hospital, University of South China, Hengyang 421001, China.
Yunsheng ZhangClinical Research Center For Breast and Thyroid Disease Prevention of Hunan Province, Hengyang 421001, China.
University of South China · CNHunan Institute of Technology · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveTo study the effects of the overexpression of autophagy-related gene 3 (ATG3) on autophagy and salinomycin-induced apoptosis in breast cancer cells and explore the underlying mechanisms.

methodsWe used the lentivirus approach to establish a breast cancer cell line with stable overexpression of ATG3. Western blotting, immunofluorescence staining and transmission electron microscopy were used to analyze the effect of ATG3 overexpression on autophagy in breast cancer MCF-7 cells. Using the AKT/mTOR agonists SC79 and MHY1485, we analyzed the effect of AKT/mTOR signal pathway activation on ATG3 overexpression-induced autophagy. Western blotting and flow cytometry were used to analyze the effect of autophagy on apoptosis of the ATG3-overexpressing cells treated with salinomycin and 3-MA (an autophagy inhibitor).

resultsIn ATG3-overexpressing MCF-7 cells, ATG3 overexpression obviously promoted autophagy, inhibited the AKT/mTOR signaling pathway, significantly weakened salinomycin-induced apoptosis (

conclusionsATG3 overexpression promotes autophagy possibly by inhibiting the AKT/mTOR signaling pathway to decrease salinomycin-induced apoptosis in MCF-7 cells, suggesting that autophagy induction might be one of the mechanisms of drug resistance in breast cancer cells.

Indexed as

AutophagyAcetatesApoptosisAutophagy-Related ProteinsBenzopyransBreast NeoplasmsCell Line, TumorCell ProliferationDrug Resistance, NeoplasmFemaleGene Expression RegulationHumansMCF-7 CellsMorpholinesPolyether PolyketidesProto-Oncogene Proteins c-akt4,6-dimorpholino-N-(4-nitrophenyl)-1,3,5-triazin-2-amineAcetatesAKT activator SC79ATG3 protein, humanAutophagy-Related ProteinsBenzopyransMorpholinesMTOR protein, humanPolyether PolyketidesProto-Oncogene Proteins c-aktPyranssalinomycinTOR Serine-Threonine KinasesTriazinesUbiquitin-Conjugating Enzymesautophagyautophagy-related gene 3breast cancerlentivirussalinomycin

Identifiers

PMID30890503
PMCPMC6765638
OpenAlexW2991619924

What Socratic holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.