ArticleTransgenic research2019
Production of transgenic cattle expressing lysine-rich polypeptide in milk by somatic cell nuclear transfer.
Article in Transgenic research, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
1 citing paper in PubMed, 9 citations in OpenAlex.
- Characterization of the regulatory 5'-flanking region of bovine mucin 2 (MUC2) gene.Molecular and cellular biochemistry · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
This study aims to produce transgenic cattle expressing lysine-rich polypeptide in milk by somatic cell nuclear transfer. Lysine is the first limiting amino acid in cereal crops. Lysine deficiency leads to fatigue, nausea and reproductive disorders. In this study, transgenic cells with lysine-rich cDNA (β-casein, αS2-casein and lactotransferrin cDNA) were used as donor cells to produce transgenic cattle by somatic cell nuclear transfer. The transgene integration and expression in transgenic cattle were examined using western blotting, Southern blotting, PCR and RT-PCR. Our results showed that the lysine-rich gene was successfully integrated into the bovine genome and expressed in the mammary gland of transgenic cattle during lactation. The milk lysine concentrations of three transgenic cattle were higher than those of non-transgenic controls. The production of transgenic cattle expressing lysine-rich polypeptides in milk may provide a large-scale and cost-effective resource for lysine-rich products.
Indexed as
Identifiers
What Socratic holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.