ArticleEuropean journal of histochemistry : EJH2019
Embedding cell monolayers to investigate nanoparticle-plasmalemma interactions at transmission electron microscopy.
Article in European journal of histochemistry : EJH, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- Mitochondrial Features of Mouse Myoblasts Are Finely Tuned by Low Doses of Ozone: The Evidence In Vitro.International journal of molecular sciences · 2023Article
- L-Carnitine Functionalization to Increase Skeletal Muscle Tropism of PLGA Nanoparticles.International journal of molecular sciences · 2022Article
- Ozone at low concentration modulates microglial activity in vitro: A multimodal microscopy and biomolecular study.Microscopy research and technique · 2022Article
- Histochemistry for nanomedicine: Novelty in tradition.European journal of histochemistry : EJH · 2021Article
- Transmission Electron Microscopy as a Powerful Tool to Investigate the Interaction of Nanoparticles with Subcellular Structures.International journal of molecular sciences · 2021Review
- Formulative Study and Intracellular Fate Evaluation of Ethosomes and Transethosomes for Vitamin D3 Delivery.International journal of molecular sciences · 2021Article
- Ethosomes and Transethosomes for Mangiferin Transdermal Delivery.Antioxidants (Basel, Switzerland) · 2021Article
- Improving the Cellular Uptake of Biomimetic Magnetic Nanoparticles.Nanomaterials (Basel, Switzerland) · 2021Article
- Twenty years of histochemistry in the third millennium, browsing the scientific literature.European journal of histochemistry : EJH · 2020Article
- Imaging techniques in nanomedical research.European journal of histochemistry : EJH · 2020Article
- Alcian blue staining to track the intracellular fate of hyaluronic-acid-based nanoparticles at transmission electron microscopy.European journal of histochemistry : EJH · 2019Article
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Authors and funding
2 authors.
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Abstract
Transmission electron microscopy is the technique of choice to visualize the spatial relationships between nanoconstructs and cells and especially to monitor the uptake process of nanomaterials. It is therefore crucial that the cell surface be preserved in its integrity, to obtain reliable ultrastructural evidence: the plasmalemma represents the biological barrier the nanomaterials have to cross, and the mode of membrane-nanoconstruct interaction is responsible for the intracellular fate of the nanomaterials. In this paper, we describe a simple and inexpensive method to process cell monolayers for ultrastructural morphology and immunocytochemistry, ensuring consistent preservation of the cell surface and of the occurring interactions with nanoparticles of different chemical composition.
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