Evidence map›Paper›PMID 30958640›Full record

ArticleCytometry. Part A : the journal of the International Society for Analytical Cytology2019

Machine Learning Based Real-Time Image-Guided Cell Sorting and Classification.

Yi Gu, Alex Ce Zhang, Yuanyuan Han, Jie Li, Clark Chen, Yu-Hwa Lo

Open access · bronzeAbstract read
In one paragraph

Article in Cytometry. Part A : the journal of the International Society for Analytical Cytology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.

0numbers the graph read from it
0cells of the map it votes in
31citing papers in PubMed
12.5field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

31 citing papers in PubMed, 84 citations in OpenAlex.

  1. Review
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  5. Article
  6. Optical sorting: past, present and future.Light, science & applications · 2025
    Review
  7. Deep Learning-Assisted Label-Free Parallel Cell Sorting with Digital Microfluidics.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025
    Article
  8. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Yi GuDepartment of Electrical and Computer Engineering, University of California, San Diego, California.ORCID 0000-0001-7962-9466
Alex Ce ZhangDepartment of Electrical and Computer Engineering, University of California, San Diego, California.
Yuanyuan HanDepartment of Electrical and Computer Engineering, University of California, San Diego, California.
Jie LiDepartment of Neurosurgery, University of Minnesota, Minneapolis, Minnesota.
Clark ChenDepartment of Neurosurgery, University of Minnesota, Minneapolis, Minnesota.
Yu-Hwa LoDepartment of Electrical and Computer Engineering, University of California, San Diego, California.
University of California San Diego · USUniversity of Minnesota · US

Funding

Targeting Mechanisms of Acquired Temozolomide Resistance in GlioblastomaR01NS097649 · NINDS · UNIVERSITY OF MINNESOTA · PI CHEN, CLARK CHIN-CHUNG · 2016 to 2021
$1.7M
iFACS: Imaging Florescence Activated Cell Sorter to sort cells based on imagesR44DA042636 · NIDA · NANOCELLECT BIOMEDICAL, INC. · PI CHO, SUNG HWAN, LO, YU-HWA · 2017 to 2018
$1.5M
3D-FACS: 3D image-based fluorescence activated cell sortingR44DA045460 · NIDA · NANOCELLECT BIOMEDICAL, INC. · PI CHO, SUNG HWAN, LO, YU-HWA · 2019 to 2020
$1.5M
NIDA NIH HHS R44 DA042636NIDA NIH HHS R44 DA045460NINDS NIH HHS R01 NS097649
6 · The paper itself

Abstract

Cell classification based on phenotypical, spatial, and genetic information greatly advances our understanding of the physiology and pathology of biological systems. Technologies derived from next generation sequencing and fluorescent activated cell sorting are cornerstones for cell- and genomic-based assays supporting cell classification and mapping. However, there exists a deficiency in technology space to rapidly isolate cells based on high content image information. Fluorescence-activated cell sorting can only resolve cell-to-cell variation in fluorescence and optical scattering. Utilizing microfluidics, photonics, computation microscopy, real-time image processing and machine learning, we demonstrate an image-guided cell sorting and classification system possessing the high throughput of flow cytometer and high information content of microscopy. We demonstrate the utility of this technology in cell sorting based on (1) nuclear localization of glucocorticoid receptors, (2) particle binding to the cell membrane, and (3) DNA damage induced γ-H2AX foci. © 2019 International Society for Advancement of Cytometry.

Indexed as

Computer SystemsMachine LearningAlgorithmsAnimalsCell Line, TumorCell MembraneDogsFlow CytometryGamma RaysGreen Fluorescent ProteinsHumansMadin Darby Canine Kidney CellsProteinsGreen Fluorescent ProteinsProteinsimage guided cell sortingimaging flow cytometrymachine learningmicrofluidic

Identifiers

PMID30958640
PMCPMC6520141
OpenAlexW2939345579

What Socratic holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.