Evidence map›Paper›PMID 31014396›Full record

ArticleGenome medicine2019

TCF21 and AP-1 interact through epigenetic modifications to regulate coronary artery disease gene expression.

Quanyi Zhao, Robert Wirka, Trieu Nguyen, Manabu Nagao, Paul Cheng, Clint L Miller, Juyong Brian Kim, Milos Pjanic, Thomas Quertermous

Open access · goldAbstract read
In one paragraph

Article in Genome medicine, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
35citing papers in PubMed, 1 pooled it
3.1field-weighted citation impact, top 7% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

35 citing papers in PubMed, 1 synthesis or guideline pooled it, 59 citations in OpenAlex.

  1. Pooled it
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  7. Susceptibility ofHeliyon · 2024
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  9. International journal of medical sciences · 2024
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 1 country.

Quanyi ZhaoDivision of Cardiovascular Medicine and Cardiovascular Institute, School of Medicine, Stanford University, 300 Pasteur Dr., Falk CVRC, Stanford, CA, 94305, USA.
Robert WirkaDivision of Cardiovascular Medicine and Cardiovascular Institute, School of Medicine, Stanford University, 300 Pasteur Dr., Falk CVRC, Stanford, CA, 94305, USA.
Trieu NguyenDivision of Cardiovascular Medicine and Cardiovascular Institute, School of Medicine, Stanford University, 300 Pasteur Dr., Falk CVRC, Stanford, CA, 94305, USA.
Manabu NagaoDivision of Cardiovascular Medicine and Cardiovascular Institute, School of Medicine, Stanford University, 300 Pasteur Dr., Falk CVRC, Stanford, CA, 94305, USA.
Paul ChengDivision of Cardiovascular Medicine and Cardiovascular Institute, School of Medicine, Stanford University, 300 Pasteur Dr., Falk CVRC, Stanford, CA, 94305, USA.
Clint L MillerCenter for Public Health Genomics, Department of Public Health Sciences, University of Virginia, Charlottesville, VA, 22908, USA.
Juyong Brian KimDivision of Cardiovascular Medicine and Cardiovascular Institute, School of Medicine, Stanford University, 300 Pasteur Dr., Falk CVRC, Stanford, CA, 94305, USA.
Milos PjanicDivision of Cardiovascular Medicine and Cardiovascular Institute, School of Medicine, Stanford University, 300 Pasteur Dr., Falk CVRC, Stanford, CA, 94305, USA.
Thomas QuertermousDivision of Cardiovascular Medicine and Cardiovascular Institute, School of Medicine, Stanford University, 300 Pasteur Dr., Falk CVRC, Stanford, CA, 94305, USA. tomq1@stanford.edu.
Cardiovascular Institute of the South · USStanford University · USUniversity of Virginia Health System · US

Funding

Stanford Islet Research CoreP30DK116074 · NIDDK · STANFORD UNIVERSITY · PI HASSAN CHAIB · 2017 to 2026
$19.5M
Causal variant association mechanisms in TCF21 binding coronary disease lociR01HL134817 · NHLBI · STANFORD UNIVERSITY · PI THOMAS QUERTERMOUS · 2017 to 2026
$6.2M
Mechanism of the Coronary Heart Disease Association at Chromosome 6q23.2R01HL109512 · NHLBI · STANFORD UNIVERSITY · PI QUERTERMOUS, THOMAS · 2011 to 2019
$6.1M
The SMAD3 signaling network in coronary artery disease riskR01HL139478 · NHLBI · STANFORD UNIVERSITY · PI THOMAS QUERTERMOUS · 2018 to 2026
$3.7M
Molecular Mechanisms of Insulin Resistance Associated LociR01DK107437 · NIDDK · STANFORD UNIVERSITY · PI QUERTERMOUS, THOMAS · 2016 to 2019
$2.2M
LncRNA Transcriptional Mechanisms of Coronary Artery Disease RiskR01HL145708 · NHLBI · STANFORD UNIVERSITY · PI QUERTERMOUS, THOMAS · 2019 to 2022
$1.6M
Identification of causal coronary heart disease variation in smooth muscle cellsR33HL120757 · NHLBI · STANFORD UNIVERSITY · PI QUERTERMOUS, THOMAS · 2016 to 2018
$1.6M
From Locus to Function: Role of ZEB2 in Human Risk of Coronary Artery DiseaseK08HL153798 · NHLBI · STANFORD UNIVERSITY · PI CHENG, PAUL PO SHENG · 2020 to 2024
$933k
Regulation of Inflammation and Atherosclerosis by TCF21K08HL133375 · NHLBI · STANFORD UNIVERSITY · PI KIM, JUYONG BRIAN · 2016 to 2020
$840k
EPISTATIC REGULATORY MECHANISMS OF CORONARY HEART DISEASE RISKR00HL125912 · NHLBI · UNIVERSITY OF VIRGINIA · PI MILLER, CLINT L · 2017 to 2019
$747k
The molecular mechanisms of SMAD3-mediated coronary disease riskF32HL143847 · NHLBI · STANFORD UNIVERSITY · PI CHENG, PAUL PO SHENG · 2018 to 2019
$114k
NHLBI NIH HHS F32 HL143847NHLBI NIH HHS K08 HL133375NHLBI NIH HHS K08 HL153798NHLBI NIH HHS R00 HL125912NHLBI NIH HHS R00HL125912NHLBI NIH HHS R01 HL109512NHLBI NIH HHS R01HL109512NHLBI NIH HHS R01 HL134817NHLBI NIH HHS R01HL134817NHLBI NIH HHS R01 HL139478NHLBI NIH HHS R01HL139478NHLBI NIH HHS R01 HL145708NHLBI NIH HHS R01HL145708NHLBI NIH HHS R33 HL120757NHLBI NIH HHS R33HL120757NIDDK NIH HHS P30 DK116074NIDDK NIH HHS R01 DK107437NIDDK NIH HHS R01DK107437
6 · The paper itself

Abstract

backgroundGenome-wide association studies have identified over 160 loci that are associated with coronary artery disease. As with other complex human diseases, risk in coronary disease loci is determined primarily by altered expression of the causal gene, due to variation in binding of transcription factors and chromatin-modifying proteins that directly regulate the transcriptional apparatus. We have previously identified a coronary disease network downstream of the disease-associated transcription factor TCF21, and in work reported here extends these studies to investigate the mechanisms by which it interacts with the AP-1 transcription complex to regulate local epigenetic effects in these downstream coronary disease loci.

methodsGenomic studies, including chromatin immunoprecipitation sequencing, RNA sequencing, and protein-protein interaction studies, were performed in human coronary artery smooth muscle cells.

resultsWe show here that TCF21 and JUN regulate expression of two presumptive causal coronary disease genes, SMAD3 and CDKN2B-AS1, in part by interactions with histone deacetylases and acetyltransferases. Genome-wide TCF21 and JUN binding is jointly localized and particularly enriched in coronary disease loci where they broadly modulate H3K27Ac and chromatin state changes linked to disease-related processes in vascular cells. Heterozygosity at coronary disease causal variation, or genome editing of these variants, is associated with decreased binding of both JUN and TCF21 and loss of expression in cis, supporting a transcriptional mechanism for disease risk.

conclusionsThese data show that the known chromatin remodeling and pioneer functions of AP-1 are a pervasive aspect of epigenetic control of transcription, and thus, the risk in coronary disease-associated loci, and that interaction of AP-1 with TCF21 to control epigenetic features, contributes to the genetic risk in loci where they co-localize.

Indexed as

Chromatin Assembly and DisassemblyEpigenesis, GeneticBasic Helix-Loop-Helix ProteinsCells, CulturedCoronary Artery DiseaseCyclin-Dependent Kinase Inhibitor p15HEK293 CellsHumansSmad3 ProteinTranscription Factor AP-1Basic Helix-Loop-Helix ProteinsCDKN2B protein, humanCyclin-Dependent Kinase Inhibitor p15Smad3 ProteinSMAD3 protein, humanTCF21 protein, humanTranscription Factor AP-1AP-1DeacetylaseEpigenomicsHistone acetyltransferaseTCF21Transcription

Identifiers

PMID31014396
PMCPMC6480881
OpenAlexW2945485301

What Socratic holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.