Evidence map›Paper›PMID 31024337›Full record

ArticleFrontiers in physiology2019

A Short-Term High-Fat Diet Alters Glutathione Levels and IL-6 Gene Expression in Oxidative Skeletal Muscles of Young Rats.

David E Andrich, Lilya Melbouci, Ya Ou, Nickolas Auclair, Jocelyne Mercier, Jean-Christophe Grenier, Fábio Santos Lira, Luis B Barreiro, Gawiyou Danialou, Alain-Steve Comtois and 2 more

Open access · goldAbstract read
In one paragraph

Article in Frontiers in physiology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.

0numbers the graph read from it
0cells of the map it votes in
21citing papers in PubMed
2.2field-weighted citation impact, top 12% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

21 citing papers in PubMed, 39 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 4 institutions in 2 countries.

David E AndrichDépartement des Sciences de l'Activité Physique, Université du Québec à Montréal (UQAM), Montréal, QC, Canada.
Lilya MelbouciDépartement des Sciences de l'Activité Physique, Université du Québec à Montréal (UQAM), Montréal, QC, Canada.
Ya OuDépartement des Sciences de l'Activité Physique, Université du Québec à Montréal (UQAM), Montréal, QC, Canada.
Nickolas AuclairDépartement des Sciences de l'Activité Physique, Université du Québec à Montréal (UQAM), Montréal, QC, Canada.
Jocelyne MercierDépartement des Sciences de l'Activité Physique, Université du Québec à Montréal (UQAM), Montréal, QC, Canada.
Jean-Christophe GrenierCentre de Recherche du CHU Sainte-Justine, Montréal, QC, Canada.
Fábio Santos LiraDépartement des Sciences de l'Activité Physique, Université du Québec à Montréal (UQAM), Montréal, QC, Canada.
Luis B BarreiroCentre de Recherche du CHU Sainte-Justine, Montréal, QC, Canada.
Gawiyou DanialouDépartement des Sciences de l'Activité Physique, Université du Québec à Montréal (UQAM), Montréal, QC, Canada.
Alain-Steve ComtoisDépartement des Sciences de l'Activité Physique, Université du Québec à Montréal (UQAM), Montréal, QC, Canada.
Jean-Claude LavoieCentre de Recherche du CHU Sainte-Justine, Montréal, QC, Canada.
David H St-PierreDépartement des Sciences de l'Activité Physique, Université du Québec à Montréal (UQAM), Montréal, QC, Canada.
Centre Hospitalier Universitaire Sainte-Justine · CAUniversité du Québec à Montréal · CARoyal Military College Saint-Jean · CAUniversidade Estadual Paulista (Unesp) · BR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Obesity and ensuing disorders are increasingly prevalent worldwide. High-fat diets (HFD) and diet-induced obesity have been shown to induce oxidative stress and inflammation while altering metabolic homeostasis in many organs, including the skeletal muscle. We previously observed that 14 days of HFD impairs contractile functions of the soleus (SOL) oxidative skeletal muscle. However, the mechanisms underlying these effects are not clarified. In order to determine the effects of a short-term HFD on skeletal muscle glutathione metabolism, young male Wistar rats (100-125 g) were fed HFD or a regular chow diet (RCD) for 14 days. Reduced (GSH) and disulfide (GSSG) glutathione levels were measured in the SOL. The expression of genes involved in the regulation of glutathione metabolism, oxidative stress, antioxidant defense and inflammation were measured by RNA-Seq. We observed a significant 25% decrease of GSH levels in the SOL muscle. Levels of GSSG and the GSH:GSSG ratio were similar in both groups. Further, we observed a 4.5 fold increase in the expression of pro-inflammatory cytokine interleukin 6 (IL-6) but not of other cytokines or markers of inflammation and oxidative stress. We hereby demonstrate that a short-term HFD significantly lowers SOL muscle GSH levels. This effect could be mediated through the increased expression of IL-6. Further, the skeletal muscle antioxidant defense could be impaired under cellular stress. We surmise that these early alterations could contribute to HFD-induced insulin resistance observed in longer protocols.

Indexed as

gene expressionhigh-fat dietinflammationmuscle glutathioneoxidative stressyoung rats

Identifiers

PMID31024337
PMCPMC6468044
OpenAlexW2936076695

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.