Evidence map›Paper›PMID 31138136›Full record

Trial reportBMC geriatrics2019

No effect of 25-hydroxyvitamin D supplementation on the skeletal muscle transcriptome in vitamin D deficient frail older adults.

Roland W J Hangelbroek, Anouk M M Vaes, Mark V Boekschoten, Lex B Verdijk, Guido J E J Hooiveld, Luc J C van Loon, Lisette C P G M de Groot, Sander Kersten

Registry-linked trialOpen access · goldAbstract readRandomized Controlled Trial
PubMed Full text at PMC Publisher
In one paragraph

Trial report in BMC geriatrics, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT02349282 (The Effect of Calcifediol), which is not on this map. Cited by 10 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed, 1 pooled it
2.0field-weighted citation impact, top 14% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

NCT02349282 nacompletednot on this map

The Effect of Calcifediol (Hy.D 25 SD/S) and Vitamin D3 on Muscle Strength in a Frail Elderly Population: a Randomized, Double-blind, Placebo-controlled Trial.

TypeinterventionalSponsorWageningen UniversityRan2014 to 2015Enrolled78ConditionsDeficiency, Vitamin D, Elderly, FrailArmsCalcifediol 10ug/day, Vitamin D3 20ug/day, Placebo
3 · Its place in the literature

Who cites it

10 citing papers in PubMed, 1 synthesis or guideline pooled it, 22 citations in OpenAlex.

  1. Pooled it
  2. Trial
  3. Trial
  4. Vitamin DJournal of cachexia, sarcopenia and muscle · 2021
    Trial
  5. 25-Hydroxyvitamin DScientific reports · 2026
    Article
  6. Article
  7. Review
  8. Article
  9. Article
  10. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 1 country.

Roland W J HangelbroekTop Institute Food and Nutrition, P.O. Box 557, 6700, AN, Wageningen, The Netherlands.
Anouk M M VaesTop Institute Food and Nutrition, P.O. Box 557, 6700, AN, Wageningen, The Netherlands.
Mark V BoekschotenTop Institute Food and Nutrition, P.O. Box 557, 6700, AN, Wageningen, The Netherlands.
Lex B VerdijkTop Institute Food and Nutrition, P.O. Box 557, 6700, AN, Wageningen, The Netherlands.
Guido J E J HooiveldTop Institute Food and Nutrition, P.O. Box 557, 6700, AN, Wageningen, The Netherlands.
Luc J C van LoonTop Institute Food and Nutrition, P.O. Box 557, 6700, AN, Wageningen, The Netherlands.
Lisette C P G M de GrootTop Institute Food and Nutrition, P.O. Box 557, 6700, AN, Wageningen, The Netherlands.
Sander KerstenTop Institute Food and Nutrition, P.O. Box 557, 6700, AN, Wageningen, The Netherlands. sander.kersten@wur.nl.ORCID 0000-0003-4488-7734
TiFN · NLMaastricht University Medical Centre · NL

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveVitamin D deficiency is common among older adults and has been linked to muscle weakness. Vitamin D supplementation has been proposed as a strategy to improve muscle function in older adults. The aim of this study was to investigate the effect of calcifediol (25-hydroxycholecalciferol) on whole genome gene expression in skeletal muscle of vitamin D deficient frail older adults.

methodsA double-blind placebo-controlled trial was conducted in vitamin D deficient frail older adults (aged above 65), characterized by blood 25-hydroxycholecalciferol concentrations between 20 and 50 nmol/L. Subjects were randomized across the placebo group and the calcifediol group (10 μg per day). Muscle biopsies were obtained before and after 6 months of calcifediol (n = 10) or placebo (n = 12) supplementation and subjected to whole genome gene expression profiling using Affymetrix HuGene 2.1ST arrays.

resultsExpression of the vitamin D receptor gene was virtually undetectable in human skeletal muscle biopsies, with Ct values exceeding 30. Blood 25-hydroxycholecalciferol levels were significantly higher after calcifediol supplementation (87.3 ± 20.6 nmol/L) than after placebo (43.8 ± 14.1 nmol/L). No significant difference between treatment groups was observed on strength outcomes. The whole transcriptome effects of calcifediol and placebo were very weak, as indicated by the fact that correcting for multiple testing using false discovery rate did not yield any differentially expressed genes using any reasonable cut-offs (all q-values ~ 1). P-values were uniformly distributed across all genes, suggesting that low p-values are likely to be false positives. Partial least squares-discriminant analysis and principle component analysis was unable to separate treatment groups.

conclusionCalcifediol supplementation did not significantly affect the skeletal muscle transcriptome in frail older adults. Our findings indicate that vitamin D supplementation has no effects on skeletal muscle gene expression, suggesting that skeletal muscle may not be a direct target of vitamin D in older adults.

trial registrationThis study was registered at clinicaltrials.gov as NCT02349282 on January 28, 2015.

Indexed as

Dietary SupplementsFrail ElderlyAgedDouble-Blind MethodFemaleHumansMaleMuscle, SkeletalTranscriptomeTreatment OutcomeVitamin DVitamin D Deficiency25-hydroxyvitamin DVitamin D25-hydroxyvitamin Dolder adultsskeletal muscletranscriptomicsVitamin D

Identifiers

PMID31138136
PMCPMC6540468
OpenAlexW2948011235

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.