ReviewPlant cell reports2019
Production of recombinant proteins through sequestration in chloroplasts: a strategy based on nuclear transformation and post-translational protein import.
Review in Plant cell reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
16 citing papers in PubMed, 23 citations in OpenAlex.
- Bt agave: why it is time to explore a new biotechnological frontier.Pest management science · 2026Review
- High-efficiency leucoplast transit peptides for manipulating plastid protein production.Nature plants · 2025Article
- Expression of an antimicrobial peptide persulcatusin fused with calmodulin in rice cultured cells.Transgenic research · 2025Article
- Targeting signals required for protein sorting to sub-chloroplast compartments.Plant cell reports · 2024Review
- Stress on the Endoplasmic Reticulum Impairs the Photosynthetic Efficiency of Chlamydomonas.International journal of molecular sciences · 2024Article
- Article
- Optimizing Promoters and Subcellular Localization for Constitutive Transgene Expression in Marchantia polymorpha.Plant & cell physiology · 2024Article
- Utilizing Plant Protein Secretion System for Pharmaceutical Protein Production.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Advances in Subcellular Accumulation Design for Recombinant Protein Production in Tobacco.Biodesign research · 2024Review
- Article
- Increasing the Efficiency of the Accumulation of Recombinant Proteins in Plant Cells: The Role of Transport Signal Peptides.Plants (Basel, Switzerland) · 2022Review
- Recombinant production of a functional SARS-CoV-2 spike receptor binding domain in the green algae Chlamydomonas reinhardtii.PloS one · 2021Article
- Production ofFrontiers in plant science · 2021Article
- High-level production in a plant system of a thermostable carbonic anhydrase and its immobilization on microcrystalline cellulose beads for COPlant cell reports · 2020Article
- Overexpression and Purification ofFrontiers in plant science · 2020Article
- Plastid biogenesis and homeostasis.Plant cell reports · 2019Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 3 institutions in 1 country.
Funding
Abstract
Recently, plants have emerged as a lucrative alternative system for the production of recombinant proteins, as recombinant proteins produced in plants are safer and cheaper than those produced in bacteria and animal cell-based production systems. To obtain high yields in plants, recombinant proteins are produced in chloroplasts using different strategies. The first strategy is based on chloroplast transformation, followed by gene expression and translation in chloroplasts. This has proven to be a powerful approach for the production of proteins at high levels. The second approach is based on nuclear transformation, followed by post-translational import of proteins from the cytosol into chloroplasts. In the nuclear transformation approach, foreign genes are stably integrated into the nuclear genome or transiently expressed in the nucleus by non-integrating T-DNA. Although this approach also has great potential for protein production at high levels, it has not been thoroughly investigated. In this review, we focus on nuclear transformation-based protein expression and its subsequent sequestration in chloroplasts, and summarize the different strategies used for high-level production of recombinant proteins. We also discuss future directions for further improvements in protein production in chloroplasts through nuclear transformation-based gene expression.
Indexed as
Identifiers
What Socratic holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.