ArticleBMC plant biology2019
Arabidopsis thaliana organelles mimic the T7 phage DNA replisome with specific interactions between Twinkle protein and DNA polymerases Pol1A and Pol1B.
Article in BMC plant biology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 28 citations in OpenAlex.
- Translesion DNA synthesis on pyrimidine dimers by plant organellar DNA polymerases is metal-dependent.Nucleic acids research · 2026Article
- Sequencing, Assembly, and Comparative Evolutionary Analysis of the Chloroplast Genome of Kenaf (Genes · 2025Article
- Not only the top: Type I topoisomerases function in multiple tissues and organs development in plants.Journal of advanced research · 2025Review
- PEN1 catalyses RNA primer removal during plastid DNA replication in maize.Nature plants · 2025Article
- Comparative analysis of chloroplast genomes in three Araceae species: genomic difference, genetic distance and species morphology association.Frontiers in genetics · 2025Article
- The evolutionarily diverged single-stranded DNA-binding proteins SSB1/SSB2 differentially affect the replication, recombination and mutation of organellar genomes inPlant diversity · 2025Article
- Primase promotes the competition between transcription and replication on the same template strand resulting in DNA damage.Nature communications · 2024Article
- The plant organellar primase-helicase directs template recognition and primosome assembly via its zinc finger domain.BMC plant biology · 2023Article
- Article
- Comparative transcriptome analysis identifiedFrontiers in plant science · 2022Article
- Comparative Analysis of the Chloroplast Genome forFrontiers in genetics · 2022Article
- Arabidopsis thaliana PrimPol is a primase and lesion bypass DNA polymerase with the biochemical characteristics to cope with DNA damage in the nucleus, mitochondria, and chloroplast.Scientific reports · 2021Article
- Construction of a highly error-prone DNA polymerase for developing organelle mutation systems.Nucleic acids research · 2020Article
- Review
- The replication machinery of LUCA: common origin of DNA replication and transcription.BMC biology · 2020Article
- DNA Repair and the Stability of the Plant Mitochondrial Genome.International journal of molecular sciences · 2020Review
- Structure-Function Analysis Reveals the Singularity of Plant Mitochondrial DNA Replication Components: A Mosaic and Redundant System.Plants (Basel, Switzerland) · 2019Review
- Plant DNA Polymerases.International journal of molecular sciences · 2019Review
- Plant Organelle Genome Replication.Plants (Basel, Switzerland) · 2019Review
- A Bacteriophage-Derived Primase-Helicase Orchestrates Plant Organellar DNA Replication.Physiologia plantarumArticle
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Authors and funding
9 authors at 2 institutions in 2 countries.
Funding
Abstract
backgroundPlant chloroplasts and mitochondria utilize nuclear encoded proteins to replicate their DNA. These proteins are purposely built for replication in the organelle environment and are distinct from those involved in replication of the nuclear genome. These organelle-localized proteins have ancestral roots in bacterial and bacteriophage genes, supporting the endosymbiotic theory of their origin. We examined the interactions between three of these proteins from Arabidopsis thaliana: a DNA helicase-primase similar to bacteriophage T7 gp4 protein and animal mitochondrial Twinkle, and two DNA polymerases, Pol1A and Pol1B. We used a three-pronged approach to analyze the interactions, including Yeast-two-hybrid analysis, Direct Coupling Analysis (DCA), and thermophoresis.
resultsYeast-two-hybrid analysis reveals residues 120-295 of Twinkle as the minimal region that can still interact with Pol1A or Pol1B. This region is a part of the primase domain of the protein and slightly overlaps the zinc-finger and RNA polymerase subdomains located within. Additionally, we observed that Arabidopsis Twinkle interacts much more strongly with Pol1A versus Pol1B. Thermophoresis also confirms that the primase domain of Twinkle has higher binding affinity than any other region of the protein. Direct-Coupling-Analysis identified specific residues in Twinkle and the DNA polymerases critical to positive interaction between the two proteins.
conclusionsThe interaction of Twinkle with Pol1A or Pol1B mimics the minimal DNA replisomes of T7 phage and those present in mammalian mitochondria. However, while T7 and mammals absolutely require their homolog of Twinkle DNA helicase-primase, Arabidopsis Twinkle mutants are seemingly unaffected by this loss. This implies that while Arabidopsis mitochondria mimic minimal replisomes from T7 and mammalian mitochondria, there is an extra level of redundancy specific to loss of Twinkle function.
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