Evidence mapPaperPMID 31327157Full record

ArticleEndocrine2019

Activation of retinal Müller cells in response to glucose variability.

Fabiana Picconi, Mariacristina Parravano, Francesca Sciarretta, Chiara Fulci, Michela Nali, Simona Frontoni, Monica Varano, Anna Maria Caccuri

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Article in Endocrine, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
30citing papers in PubMed, 1 pooled it
field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

30 citing papers in PubMed, 1 synthesis or guideline pooled it.

  1. Pooled it
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  5. Effect of ranibizumab on diabetic retinopathyWorld journal of diabetes · 2025
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  8. Cells · 2024
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Fabiana PicconiDepartment of Systems Medicine, University of Rome Tor Vergata, Rome, Italy.
Mariacristina ParravanoIRCCS-Fondazione Bietti, Rome, Italy.
Francesca SciarrettaDepartment of Experimental Medicine, University of Rome Tor Vergata, Rome, Italy.
Chiara FulciDepartment of Experimental Medicine, University of Rome Tor Vergata, Rome, Italy.
Michela NaliDepartment of Experimental Medicine, University of Rome Tor Vergata, Rome, Italy.
Simona FrontoniDepartment of Systems Medicine, University of Rome Tor Vergata, Rome, Italy. frontoni@uniroma2.it.ORCID 0000-0001-8241-7552
Monica VaranoIRCCS-Fondazione Bietti, Rome, Italy.
Anna Maria CaccuriDepartment of Experimental Medicine, University of Rome Tor Vergata, Rome, Italy.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

purposeIn the earliest stages of diabetic retinopathy (DR), a dysfunction of Müller cells, characterized by high levels of glial fibrillary acidic protein (GFAP), and aquaporins (AQP), has been observed. Although chronic hyperglycemia causes the activation of Müller cells, the effect of glycemic fluctuations is yet unknown. The aim of the study was to analyze the impact of glucose variability on rat retinal Müller cells (rMC-1) adapted to either normal (5 mM) or high (25 mM) glucose levels.

methodsrMC-1 were cultured in a medium containing either 5 mM (N cells) or 25 mM of glucose (H cells) and then incubated for 96 h in a medium containing (a) low glucose (either 1-3 or 5 mM), (b) basal glucose (either 5 or 25 mM), (c) high glucose (either 25 or 45 mM), (d) basal and high glucose alternated every 24 h; (e) low- and high glucose alternated every 24 h; (f) basal glucose with episodes of low glucose for 30 min twice a day. Müller cells activation was evaluated by measuring the levels of GFAP, AQP4, and phospho-active extracellular signal-regulated kinase (pERK).

resultsUnder both basal and high glucose concentrations rMC-1 were viable, but their response to glucose excursions was different. In N cells kept under normal (5 mM) glucose, a significant glial activation was measured not only in response to constant high glucose but also to alternating low/high glucose. In H cells, adapted to 25 mM glucose, a significant response was observed only after exposition to a lower (5 mM) glucose concentration.

conclusionOur results highlight Müller cells activation in response to glucose variability and a different susceptibility depending on the basal glucose conditions.

Indexed as

AnimalsAquaporin 4Cell CycleCells, CulturedDiabetic RetinopathyEpendymoglial CellsExtracellular Signal-Regulated MAP KinasesGlial Fibrillary Acidic ProteinGlucoseHyperglycemiaNeurogliaRatsRetinaAqp4 protein, ratAquaporin 4Extracellular Signal-Regulated MAP KinasesGfap protein, ratGlial Fibrillary Acidic ProteinGlucoseGlucose variabilityMüller cells activationRetinal neurodegeneration

Identifiers

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.