Evidence map›Paper›PMID 31331998›Full record

ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2019

Differential Signaling Mediated by ApoE2, ApoE3, and ApoE4 in Human Neurons Parallels Alzheimer's Disease Risk.

Yu-Wen Alvin Huang, Bo Zhou, Amber M Nabet, Marius Wernig, Thomas C Südhof

Open access · bronzeAbstract read
In one paragraph

Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 92 papers, 4 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
92citing papers in PubMed, 4 pooled it
8.9field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

92 citing papers in PubMed, 4 syntheses or guidelines pooled it, 152 citations in OpenAlex.

  1. Pooled it
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  5. Review
  6. TREM2 in neurodegeneration and diseases.Molecular psychiatry · 2026
    Review
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  16. Concentrations of the serum long-chain omega-3 polyunsaturated fatty acids and hair mercury in men in different apolipoprotein E phenotypes.Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine · 2025
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32 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 2 countries.

Yu-Wen Alvin HuangDepartment of Molecular and Cellular Physiology and Howard Hughes Medical Institute, Stanford University Medical School, Stanford, California 94305, alvinhuang@brown.edu tcs1@stanford.edu.
Bo ZhouDepartment of Molecular and Cellular Physiology and Howard Hughes Medical Institute, Stanford University Medical School, Stanford, California 94305.
Amber M NabetDepartment of Molecular and Cellular Physiology and Howard Hughes Medical Institute, Stanford University Medical School, Stanford, California 94305.ORCID 0000-0003-2582-3997
Marius WernigInstitute for Stem Cell Biology and Regenerative Medicine and Department of Pathology, Stanford University Medical School, Stanford, California 94305.
Thomas C SüdhofDepartment of Molecular and Cellular Physiology and Howard Hughes Medical Institute, Stanford University Medical School, Stanford, California 94305, alvinhuang@brown.edu tcs1@stanford.edu.
Howard Hughes Medical Institute · USCalifornia Institute for Regenerative Medicine · US

Funding

Probing Alzheimer synaptopathy in neurons derived from engineered human iPS cellsRF1AG048131 · NIA · STANFORD UNIVERSITY · PI SUDHOF, THOMAS C., WERNIG, MARIUS · 2014 to 2019
$5.4M
A Molecular Signaling Pathway Underlying Differential Predisposition of ApoE4 Genotype to Alzheimer's DiseaseR00AG054616 · NIA · BROWN UNIVERSITY · PI HUANG, YU-WEN ALVIN · 2019 to 2021
$722k
A molecular signaling pathway underlying differential predisposition of ApoE4 genotype to Alzheimer's diseaseK99AG054616 · NIA · STANFORD UNIVERSITY · PI HUANG, YU-WEN ALVIN · 2017 to 2018
$237k
Howard Hughes Medical InstituteNIA NIH HHS K99 AG054616NIA NIH HHS R00 AG054616NIA NIH HHS RF1 AG048131
6 · The paper itself

Abstract

In blood, apolipoprotein E (ApoE) is a component of circulating lipoproteins and mediates the clearance of these lipoproteins from blood by binding to ApoE receptors. Humans express three genetic ApoE variants, ApoE2, ApoE3, and ApoE4, which exhibit distinct ApoE receptor-binding properties and differentially affect Alzheimer's disease (AD), such that ApoE2 protects against, and ApoE4 predisposes to AD. In brain, ApoE-containing lipoproteins are secreted by activated astrocytes and microglia, but their functions and role in AD pathogenesis are largely unknown. Ample evidence suggests that ApoE4 induces microglial dysregulation and impedes Aβ clearance in AD, but the direct neuronal effects of ApoE variants are poorly studied. Extending previous studies, we here demonstrate that the three ApoE variants differentially activate multiple neuronal signaling pathways and regulate synaptogenesis. Specifically, using human neurons (male embryonic stem cell-derived) cultured in the absence of glia to exclude indirect glial mechanisms, we show that ApoE broadly stimulates signal transduction cascades. Among others, such stimulation enhances APP synthesis and synapse formation with an ApoE4>ApoE3>ApoE2 potency rank order, paralleling the relative risk for AD conferred by these ApoE variants. Unlike the previously described induction of

Indexed as

Alzheimer DiseaseAnimalsAnimals, NewbornApolipoprotein E2Apolipoprotein E3Apolipoprotein E4Cells, CulturedDouble-Blind MethodEmbryonic Stem CellsFemaleGenetic Predisposition to DiseaseGenetic VariationHEK293 CellsHumansMaleMiceApolipoprotein E2Apolipoprotein E3Apolipoprotein E4Alzheimer's disease (AD)apolipoprotein E (ApoE)induced neuronal (iN) cellssignaling pathwaysynapse formation

Identifiers

PMID31331998
PMCPMC6759032
OpenAlexW2962887615

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.