ArticleFrontiers in plant science2019
Toward Mass Spectrometry Imaging in the Metabolomics Scale: Increasing Metabolic Coverage Through Multiple On-Tissue Chemical Modifications.
Article in Frontiers in plant science, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed.
- Beyond the Fixed Charge: A New On-Tissue Derivatisation Strategy Employing Photoionisable Chromophores for Mass Spectrometry Imaging of Amino-Containing Metabolites.Angewandte Chemie (International ed. in English) · 2026Article
- Matrix-Assisted Laser Desorption/Ionization Imaging Mass Spectrometry.Nature reviews. Methods primers · 2026Article
- Metabolomics: Fundamentals, Methods, Analysis, Limits, and Recommendations.Advances in experimental medicine and biology · 2026Review
- Harnessing the Power of Metabolomics for Precision Oncology: Current Advances and Future Directions.Cells · 2025Review
- Mass Spectrometry Imaging of Coniine and Other Hemlock Alkaloids after On-Tissue Derivatization Reveals Distinct Alkaloid Distributions in the Plant.Journal of natural products · 2024Article
- Organ crosstalk and dysfunction in sepsis.Annals of intensive care · 2024Review
- An Analytical Evaluation of Tools for Lipid Isomer Differentiation in Imaging Mass Spectrometry.International journal of mass spectrometry · 2024Article
- Intact cell mass spectrometry coupled with machine learning reveals minute changes induced by single gene silencing.Heliyon · 2024Article
- Applications of mass spectrometry imaging in botanical research.Advanced biotechnology · 2024Review
- Article
- Improved Screening of Monoclonal Gammopathy Patients by MALDI-TOF Mass Spectrometry.Journal of the American Society for Mass Spectrometry · 2023Article
- Acidic Methanol Treatment Facilitates Matrix-Assisted Laser Desorption Ionization-Mass Spectrometry Imaging of Energy Metabolism.Analytical chemistry · 2023Article
- Autofocusing MALDI MS imaging of processed food exemplified by the contaminant acrylamide in German gingerbread.Scientific reports · 2023Article
- Article
- Spatial localization of β-unsaturated aldehyde markers in murine diabetic kidney tissue by mass spectrometry imaging.Analytical and bioanalytical chemistry · 2022Article
- On-tissue chemical derivatization in mass spectrometry imaging.Mass spectrometry reviews · 2022Review
- Advances in Plant Metabolomics and Its Applications in Stress and Single-Cell Biology.International journal of molecular sciences · 2022Review
- The application of mass spectrometry imaging in traditional Chinese medicine: a review.Chinese medicine · 2022Review
- ADVANCES IN HIGH-RESOLUTION MALDI MASS SPECTROMETRY FOR NEUROBIOLOGY.Mass spectrometry reviews · 2022Review
- Unravel the Local Complexity of Biological Environments by MALDI Mass Spectrometry Imaging.International journal of molecular sciences · 2021Review
Corrections and comments
- Erratum issued
Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Exploring the metabolic differences directly on tissues is essential for the comprehensive understanding of how multicellular organisms function. Mass spectrometry imaging (MSI) is an attractive technique toward this goal; however, MSI in metabolomics scale has been hindered by multiple limitations. This is most notable for single cell level high-spatial resolution imaging because of the limited number of molecules in small sampling size and the low ionization yields of many metabolites. Several on-tissue chemical derivatization approaches have been reported to increase MSI signals of targeted compounds, especially in matrix-assisted laser desorption/ionization (MALDI)-MSI. Herein, we adopt a combination of chemical derivatization reactions, to selectively enhance metabolite signals of a specific functional group for each consecutive tissue section. Three well-known on-tissue derivatization methods were used as a proof of concept experiment: coniferyl aldehyde for primary amines, Girard's reagent T for carbonyl groups, and 2-picolylamine for carboxylic acids. This strategy was applied to the cross-sections of leaves and roots from two different maize genotypes (B73 and Mo17), and enabled the detection of over six hundred new unique metabolite features compared to without modification. Statistical analysis indicated quantitative variation between metabolites in the tissue sections, while MS images revealed differences in localization of these metabolites. Combined, this untargeted approach facilitated the visualization of various classes of compounds, demonstrating the potential for untargeted MSI in the metabolomics scale.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.