ArticlePLoS genetics2019
Empty Pericarp21 encodes a novel PPR-DYW protein that is required for mitochondrial RNA editing at multiple sites, complexes I and V biogenesis, and seed development in maize.
Article in PLoS genetics, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed.
- Kingdom-wide evolutionary characterization of RNA editing factors in Archaeplastida.Molecular biology and evolution · 2026Article
- Pentatricopeptide repeat proteins in crops: Advances in functional mechanisms and breeding applications.Journal of integrative plant biology · 2026Review
- Combining RGB imaging with a two-stage deep learning method to reveal genetic variation of wheat sprouting traits.Plant phenomics (Washington, D.C.) · 2026Article
- Decades' progress and prospects on maize functional genomics and molecular breeding.Science China. Life sciences · 2025Review
- The first complete mitochondrial genome of Curcuma amarissima (Zingiberaceae): insights into multi-branch structure, codon usage, and phylogenetic evolution.BMC genomics · 2025Article
- Article
- Pentatricopeptide repeat proteins in plants: Cellular functions, action mechanisms, and potential applications.Plant communications · 2025Review
- Seeing the unseen in characterizing RNA editome during rice endosperm development.Communications biology · 2024Article
- Mutations in nuclear genes encoding mitochondrial ribosome proteins restore pollen fertility in S male-sterile maize.G3 (Bethesda, Md.) · 2024Article
- C1-FDX is required for the assembly of mitochondrial complex I and subcomplexes of complex V in Arabidopsis.PLoS genetics · 2024Article
- Insights into structure, codon usage, repeats, and RNA editing of the complete mitochondrial genome of Perilla frutescens (Lamiaceae).Scientific reports · 2024Article
- Multiple factors interact in editing of PPR-E+-targeted sites in maize mitochondria and plastids.Plant communications · 2024Article
- The complete mitochondrial genome ofFrontiers in plant science · 2024Article
- PPR proteins in plants: roles, mechanisms, and prospects for rice research.Frontiers in plant science · 2024Review
- The complete mitochondrial genome ofFrontiers in plant science · 2024Article
- A mitochondrial pentatricopeptide repeat protein enhances cold tolerance by modulating mitochondrial superoxide in rice.Nature communications · 2023Article
- Assembly and comparative analysis of the complete mitochondrial genome of Viburnum chinshanense.BMC plant biology · 2023Article
- A Systemic Investigation of Genetic Architecture and Gene Resources Controlling Kernel Size-Related Traits in Maize.International journal of molecular sciences · 2023Article
- Maize PPR-E proteins mediate RNA C-to-U editing in mitochondria by recruiting the trans deaminase PCW1.The Plant cell · 2023Article
- DEK48 Is Required for RNA Editing at Multiple Mitochondrial Sites and Seed Development in Maize.International journal of molecular sciences · 2022Article
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10 authors.
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Abstract
C-to-U editing is an important event in post-transcriptional RNA processing, which converts a specific cytidine (C)-to-uridine (U) in transcripts of mitochondria and plastids. Typically, the pentatricopeptide repeat (PPR) protein, which specifies the target C residue by binding to its upstream sequence, is involved in the editing of one or a few sites. Here we report a novel PPR-DYW protein EMP21 that is associated with editing of 81 sites in maize. EMP21 is localized in mitochondria and loss of the EMP21 function severely inhibits the embryogenesis and endosperm development in maize. From a scan of 35 mitochondrial transcripts produced by the Emp21 loss-of-function mutant, the C-to-U editing was found to be abolished at five sites (nad7-77, atp1-1292, atp8-437, nad3-275 and rps4-870), while reduced at 76 sites in 21 transcripts. In most cases, the failure to editing resulted in the translation of an incorrect residue. In consequence, the mutant became deficient with respect to the assembly and activity of mitochondrial complexes I and V. As six of the decreased editing sites in emp21 overlap with the affected editing sites in emp5-1, and the editing efficiency at rpl16-458 showed a substantial reduction in the emp21-1 emp5-4 double mutant compared with the emp21-1 and emp5-4 single mutants, we explored their interaction. A yeast two hybrid assay suggested that EMP21 does not interact with EMP5, but both EMP21 and EMP5 interact with ZmMORF8. Together, these results indicate that EMP21 is a novel PPR-DYW protein required for the editing of ~17% of mitochondrial target Cs, and the editing process may involve an interaction between EMP21 and ZmMORF8 (and probably other proteins).
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