ArticleNature communications2019
Degron-tagged reporters probe membrane topology and enable the specific labelling of membrane-wrapped structures.
Article in Nature communications, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed, 26 citations in OpenAlex.
- Cyclin B3-CDK1 couples translational de-repression to embryonic mitoses via OMA protein degradation inbioRxiv : the preprint server for biology · 2026Article
- The Caenorhabditis Genetics Center Curated Special Collections: a guide to protein degradation systems.Genetics · 2026Review
- Sperm activation for fertilization requires robust activity of the TAT-5 lipid flippase.Developmental biology · 2025Article
- Loss of lipid asymmetry facilitates plasma membrane blebbing by decreasing membrane lipid packing.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Dopey-dependent regulation of extracellular vesicles maintains neuronal morphology.Current biology : CB · 2024Article
- Extracellular vesicles.Genetics · 2024Review
- Phagolysosomes break down the membrane of a non-apoptotic corpse independent of macroautophagy.bioRxiv : the preprint server for biology · 2024Article
- Dopey-dependent regulation of extracellular vesicles maintains neuronal morphology.bioRxiv : the preprint server for biology · 2024Article
- Phagolysosomes break down the membrane of a non-apoptotic corpse independent of macroautophagy.PloS one · 2024Article
- The ortholog of human REEP1-4 is required for autophagosomal enclosure of ER-phagy/nucleophagy cargos in fission yeast.PLoS biology · 2023Article
- Methods for the identification and characterization of extracellular vesicles in cardiovascular studies: from exosomes to microvesicles.Cardiovascular research · 2023Article
- Visualizing Phagocytic Cargo In Vivo from Engulfment to Resolution in Caenorhabditis elegans.Methods in molecular biology (Clifton, N.J.) · 2023Article
- The ATPase activity of the phosphatidylethanolamine flippase TAT-5 inhibits extracellular vesicle budding from the plasma membrane.microPublication biology · 2023Article
- ZC3HC1 is a structural element of the nuclear basket effecting interlinkage of TPR polypeptides.Molecular biology of the cell · 2022Article
- Conserved N- and C-terminal motifs of PAD-1 are required to inhibit extracellular vesicle release.microPublication biology · 2022Article
- Article
- The power of imaging to understand extracellular vesicle biology in vivo.Nature methods · 2021Review
- A chromodomain protein mediates heterochromatin-directed piRNA expression.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Loss of the Major Phosphatidylserine or Phosphatidylethanolamine Flippases Differentially Affect Phagocytosis.Frontiers in cell and developmental biology · 2020Article
- Biological membranes in EV biogenesis, stability, uptake, and cargo transfer: an ISEV position paper arising from the ISEV membranes and EVs workshop.Journal of extracellular vesicles · 2019Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Visualization of specific organelles in tissues over background fluorescence can be challenging, especially when reporters localize to multiple structures. Instead of trying to identify proteins enriched in specific membrane-wrapped structures, we use a selective degradation approach to remove reporters from the cytoplasm or nucleus of C. elegans embryos and mammalian cells. We demonstrate specific labelling of organelles using degron-tagged reporters, including extracellular vesicles, as well as individual neighbouring membranes. These degron-tagged reporters facilitate long-term tracking of released cell debris and cell corpses, even during uptake and phagolysosomal degradation. We further show that degron protection assays can probe the topology of the nuclear envelope and plasma membrane during cell division, giving insight into protein and organelle dynamics. As endogenous and heterologous degrons are used in bacteria, yeast, plants, and animals, degron approaches can enable the specific labelling and tracking of proteins, vesicles, organelles, cell fragments, and cells in many model systems.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.