ArticleScientific reports2019
Efficient derivation of knock-out and knock-in rats using embryos obtained by in vitro fertilization.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 40 citations in OpenAlex.
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- Superovulation with an anti-inhibin monoclonal antibody improves the reproductive performance of rat strains by increasing the pregnancy rate and the litter size.Scientific reports · 2024Article
- Time elapsed between ovulation and insemination determines the quality of fertilized rat oocytes.The Journal of reproduction and development · 2024Article
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- Enhancement of rat spermatozoal hyperactivation by progesterone.The Journal of reproduction and development · 2023Article
- Feto-maternal cholesterol transport regulated by β-Klotho-FGF15 axis is essential for fetal growth.Life science alliance · 2023Article
- A combined treatment with progesterone, anti-inhibin serum, and equine chorionic gonadotropin improves number of ovulated oocytes in young C57BL/6J mice.The Journal of reproduction and development · 2023Article
- Functional calcium-responsive parathyroid glands generated using single-step blastocyst complementation.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Removal of sperm tail using trypsin and pre-activation of oocyte facilitates intracytoplasmic sperm injection in mice and rats.The Journal of reproduction and development · 2023Article
- Optimized protocols for sperm cryopreservation and in vitro fertilization in the rat.Lab animal · 2022Review
- Recent Advances in the Production of Genome-Edited Rats.International journal of molecular sciences · 2022Review
- Current status of the application of gene editing in pigs.The Journal of reproduction and development · 2021Review
- Progress of genome editing technology and developmental biology useful for radiation research.Journal of radiation research · 2021Review
- One-Step Generation of Multiple Gene-Edited Pigs by Electroporation of the CRISPR/Cas9 System into Zygotes to Reduce Xenoantigen Biosynthesis.International journal of molecular sciences · 2021Article
Corrections and comments
- Erratum issued
Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Rats are effective model animals and have contributed to the development of human medicine and basic research. However, the application of reproductive engineering techniques to rats is not as advanced compared with mice, and genome editing in rats has not been achieved using embryos obtained by in vitro fertilization (IVF). In this study, we conducted superovulation, IVF, and knock out and knock in using IVF rat embryos. We found that superovulation effectively occurred in the synchronized oestrus cycle and with anti-inhibin antiserum treatment in immature rats, including the Brown Norway rat, which is a very difficult rat strain to superovulate. Next, we collected superovulated oocytes under anaesthesia, and offspring derived from IVF embryos were obtained from all of the rat strains that we examined. When the tyrosinase gene was targeted by electroporation in these embryos, both alleles were disrupted with 100% efficiency. Furthermore, we conducted long DNA fragment knock in using adeno-associated virus and found that the knock-in litter was obtained with high efficiency (33.3-47.4%). Thus, in this study, we developed methods to allow the simple and efficient production of model rats.
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