Evidence map›Paper›PMID 31434939›Full record

Trial reportScientific reports2019

Regulation of PDE5 expression in human aorta and thoracic aortic aneurysms.

Valeriana Cesarini, Calogera Pisano, Gabriele Rossi, Carmela Rita Balistreri, Flavia Botti, Giorgio Antonelli, Giovanni Ruvolo, Emmanuele A Jannini, Susanna Dolci

Open access · goldAbstract readClinical Trial
PubMed Full text at PMC Publisher
In one paragraph

Trial report in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed
1.7field-weighted citation impact, top 14% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed, 19 citations in OpenAlex.

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  9. Love in the time of COVID-19: a scoping review on male sexual health.Journal of community hospital internal medicine perspectives · 2021
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 1 country.

Valeriana CesariniDepartment of Biomedicine and Prevention, University of Rome TorVergata, Rome, Italy.
Calogera PisanoDepartment of Surgery Sciences, University of Rome TorVergata, Rome, Italy.ORCID http://orcid.org/0000-0002-6488-1965
Gabriele RossiDepartment of Biomedicine and Prevention, University of Rome TorVergata, Rome, Italy.
Carmela Rita BalistreriDepartment of Biomedicine, Neuroscience and Advanced Diagnostics (Bi.N.D.), University of Palermo, Palermo, Italy.ORCID http://orcid.org/0000-0002-5393-1007
Flavia BottiDepartment of Clinical Sciences and Translational Medicine, University of Rome TorVergata, Rome, Italy.
Giorgio AntonelliDepartment of Biomedicine and Prevention, University of Rome TorVergata, Rome, Italy.
Giovanni RuvoloSaint Camillus International University of Health and Medical Sciences, Rome, Italy.
Emmanuele A JanniniDepartment of Systems Medicine, University of Rome TorVergata, Rome, Italy.ORCID http://orcid.org/0000-0002-5874-039X
Susanna DolciDepartment of Biomedicine and Prevention, University of Rome TorVergata, Rome, Italy. dolci@uniroma2.it.
University of Rome Tor Vergata · ITSaint Camillus International University of Health and Medical Sciences · ITUniversity of Palermo · IT

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Aneurysms and dissections affecting thoracic aorta are associated with smooth muscle cell (SMC) dysfunction. NO/cGMP signaling pathway in smooth muscle cells has been shown to be affected in sporadic thoracic aortic aneurysms. We analyzed the mRNA levels of PDE5, a cGMP-hydrolyzing enzyme highly expressed in aortic SMCs, that regulates arterious vascular tone by lowering cGMP levels. We found that aortic tissue obtained from Marfan, tricuspid and bicuspid thoracic aneurysms expressed lower levels of PDE5 mRNA compared to control aortas. In particular, we found that affected aortas showed lower levels of all the PDE5A isoforms, compared to control aortas. Transfection of vascular SMCs (VSMCs) with NOTCH3 activated domain (NICD3) induced the expression of PDE5A1 and A3 protein isoforms, but not that of the corresponding mRNAs. VSMC stimulation with GSNO, a nitric oxide analogue or with 8-br-cGMP, but not with 8-br-cAMP, up-regulated PDE5 and NOTCH-3 protein levels, indicating a negative feedback loop to protect the arterial wall from excessive relaxation. Finally, we found that PDE5 is expressed early during human aorta development, suggesting that if loss of function mutations of PDE5 occur, they might potentially affect aortic wall development.

Indexed as

Gene Expression Regulation, EnzymologicAdultAgedAortic Aneurysm, ThoracicCyclic Nucleotide Phosphodiesterases, Type 5FemaleHumansIsoenzymesMaleMiddle AgedMuscle, Smooth, VascularMyocytes, Smooth MuscleCyclic Nucleotide Phosphodiesterases, Type 5IsoenzymesPDE5A protein, human

Identifiers

PMID31434939
PMCPMC6704119
OpenAlexW2969301554

What Socratic holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.