Evidence map›Paper›PMID 31469867›Full record

ArticlePloS one2019

A pilot study on the kinetics of metabolites and microvascular cutaneous effects of nitric oxide inhalation in healthy volunteers.

Adriano R Tonelli, Kulwant S Aulak, Mostafa K Ahmed, Alfred Hausladen, Batool Abuhalimeh, Charlie J Casa, Stephen C Rogers, David Timm, Allan Doctor, Benjamin Gaston and 1 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.5field-weighted citation impact, top 27% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 4 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 7 institutions in 2 countries.

Adriano R TonelliDepartment of Pulmonary, Allergy and Critical Care Medicine, Respiratory Institute, Cleveland Clinic, Cleveland, OH, United States of America.ORCID 0000-0002-2321-9545
Kulwant S AulakInflammation and Immunity, Lerner Research Institute, Cleveland Clinic, OH, United States of America.
Mostafa K AhmedDepartment of Chest Diseases, Faculty of Medicine, Assiut University, Assiut, Egypt.
Alfred HausladenInstitute for Transformative Molecular Medicine and Department of Medicine, Case Western Reserve University School of Medicine and University Hospitals Cleveland Medical Center, Cleveland, OH, United States of America.
Batool AbuhalimehInflammation and Immunity, Lerner Research Institute, Cleveland Clinic, OH, United States of America.
Charlie J CasaInflammation and Immunity, Lerner Research Institute, Cleveland Clinic, OH, United States of America.
Stephen C RogersDepartment of Pediatrics, University of Maryland School of Medicine, Baltimore, MD, United States of America.ORCID 0000-0003-1139-4730
David TimmDepartment of Psychiatry, School of Medicine, Washington University, St. Louis, MO, United States of America.ORCID 0000-0003-1246-2707
Allan DoctorDepartment of Pediatrics, University of Maryland School of Medicine, Baltimore, MD, United States of America.
Benjamin GastonHerman B Wells Center for Pediatric Research, Indiana University School of Medicine, Indianapolis, IN, United States of America.
Raed A DweikDepartment of Pulmonary, Allergy and Critical Care Medicine, Respiratory Institute, Cleveland Clinic, Cleveland, OH, United States of America.
Cleveland Clinic Lerner College of Medicine · USCleveland Clinic · USUniversity of Maryland, Baltimore · USAssiut University · EGIndiana University School of MedicineUniversity Hospitals of Cleveland · USWashington University in St. Louis · US

Funding

Project 3 - Personalized cystic fibrosis treatment strategies with synergistic therapies Identified in vitro.P01HL128192 · NHLBI · INDIANA UNIVERSITY INDIANAPOLIS · PI GASTON, BENJAMIN · 2016 to 2020
$12.2M
SEPSIS-INDUCED RED CELL DYSFUNCTION (SIRD)R01GM113838 · NIGMS · WASHINGTON UNIVERSITY · PI DOCTOR, ALLAN, MARSHALL, GARLAND ROSS · 2015 to 2019
$4.0M
Extrapulmonary Microvascular Dysfunction in Pulmonary Arterial HypertensionR01HL130307 · NHLBI · CLEVELAND CLINIC LERNER COM-CWRU · PI TONELLI, ADRIANO · 2016 to 2020
$2.0M
NHLBI NIH HHS P01 HL128192NHLBI NIH HHS R01 HL130307NIGMS NIH HHS R01 GM113838
6 · The paper itself

Abstract

rationaleInhaled nitric oxide (NO) exerts a variety of effects through metabolites and these play an important role in regulation of hemodynamics in the body. A detailed investigation into the generation of these metabolites has been overlooked.

objectivesWe investigated the kinetics of nitrite and S-nitrosothiol-hemoglobin (SNO-Hb) in plasma derived from inhaled NO subjects and how this modifies the cutaneous microvascular response.

findingsWe enrolled 15 healthy volunteers. Plasma nitrite levels at baseline and during NO inhalation (15 minutes at 40 ppm) were 102 (86-118) and 114 (87-129) nM, respectively. The nitrite peak occurred at 5 minutes of discontinuing NO (131 (104-170) nM). Plasma nitrate levels were not significantly different during the study. SNO-Hb molar ratio levels at baseline and during NO inhalation were 4.7E-3 (2.5E-3-5.8E-3) and 7.8E-3 (4.1E-3-13.0E-3), respectively. Levels of SNO-Hb continued to climb up to the last study time point (30 min: 10.6E-3 (5.3E-3-15.5E-3)). The response to acetylcholine iontophoresis both before and during NO inhalation was inversely associated with the SNO-Hb level (r: -0.57, p = 0.03, and r: -0.54, p = 0.04, respectively).

conclusionsBoth nitrite and SNO-Hb increase during NO inhalation. Nitrite increases first, followed by a more sustained increase in Hb-SNO. Nitrite and Hb-SNO could be a mobile reservoir of NO with potential implications on the systemic microvasculature.

Indexed as

InhalationMetabolomeBiomarkersHealthy VolunteersHumansKineticsMicrocirculationMicrovesselsNitric OxidePilot ProjectsSkinBiomarkersNitric Oxide

Identifiers

PMID31469867
PMCPMC6716644
OpenAlexW2970415110

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.