Evidence map›Paper›PMID 31473160›Full record

ArticleJournal of molecular biology2019

Functional Dissection of a Viral DNA Packaging Machine's Walker B Motif.

Damian delToro, David Ortiz, Mariam Ordyan, Joshua Pajak, Jean Sippy, Alexis Catala, Choon-Seok Oh, Amber Vu, Gaurav Arya, Douglas E Smith and 2 more

Abstract read
In one paragraph

Article in Journal of molecular biology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.

0numbers the graph read from it
0cells of the map it votes in
14citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

14 citing papers in PubMed.

  1. Review
  2. Characterizing dynamics of productive dynein stepping by MINFLUX.Nature structural & molecular biology · 2026
    Article
  3. Review
  4. Article
  5. Article
  6. Article
  7. Viral Genomic DNA Packaging Machinery.Sub-cellular biochemistry · 2024
    Review
  8. Article
  9. Review
  10. Article
  11. Article
  12. Article
  13. Viral packaging ATPases utilize a glutamate switch to couple ATPase activity and DNA translocation.Proceedings of the National Academy of Sciences of the United States of America · 2021
    Article
  14. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Damian delToroDepartment of Physics, University of California, San Diego, La Jolla, CA 92093, USA.
David OrtizDepartment of Medicinal Chemistry, University of Washington, Seattle, WA 98195, USA.
Mariam OrdyanDepartment of Physics, University of California, San Diego, La Jolla, CA 92093, USA.
Joshua PajakDepartment of Mechanical Engineering and Materials Science, Duke University, Durham, NC 27708, USA.
Jean SippyDepartment of Microbiology, Roy J. and Lucille A. Carver College of Medicine, University of Iowa, Iowa City, IA 52242, USA.
Alexis CatalaDepartment of Medicinal Chemistry, University of Washington, Seattle, WA 98195, USA.
Choon-Seok OhDepartment of Microbiology, Roy J. and Lucille A. Carver College of Medicine, University of Iowa, Iowa City, IA 52242, USA.
Amber VuDepartment of Microbiology, Roy J. and Lucille A. Carver College of Medicine, University of Iowa, Iowa City, IA 52242, USA.
Gaurav AryaDepartment of Mechanical Engineering and Materials Science, Duke University, Durham, NC 27708, USA.
Douglas E SmithDepartment of Physics, University of California, San Diego, La Jolla, CA 92093, USA. Electronic address: des@ucsd.edu.
Carlos E CatalanoDepartment of Medicinal Chemistry, University of Washington, Seattle, WA 98195, USA. Electronic address: carlos.catalano@ucdenver.edu.
Michael FeissDepartment of Microbiology, Roy J. and Lucille A. Carver College of Medicine, University of Iowa, Iowa City, IA 52242, USA. Electronic address: michael-feiss@uiowa.edu.

Funding

RESEARCH TRAINING IN INFECTIOUS DISEASEST32AI007343 · NIAID · UNIVERSITY OF IOWA · PI STAPLETON, JACK T. · 1988 to 2024
$5.0M
Mechanisms of Viral DNA Packaging: Biophysical, Biochemical, & Genetic AnalysisR01GM088186 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI CATALANO, CARLOS ENRIQUE, FEISS, MICHAEL · 2011 to 2014
$2.9M
Linking Kinetics, Subunit Coordination & Structure of a Viral DNA Packaging MotorR01GM118817 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI ARYA, GAURAV, CHEMLA, YANN R. · 2016 to 2019
$1.5M
NIAID NIH HHS T32 AI007343NIGMS NIH HHS R01 GM088186NIGMS NIH HHS R01 GM118817
6 · The paper itself

Abstract

Many viruses employ ATP-powered motors for genome packaging. We combined genetic, biochemical, and single-molecule techniques to confirm the predicted Walker-B ATP-binding motif in the phage λ motor and to investigate the roles of the conserved residues. Most changes of the conserved hydrophobic residues resulted in >10

Indexed as

AAA DomainBacteriophage lambdaDNA, ViralEndodeoxyribonucleasesMolecular Dynamics SimulationMutationNucleoproteinsProtein Structure, QuaternaryViral ProteinsVirus AssemblyDNA, ViralEndodeoxyribonucleasesNucleoproteinsterminaseViral Proteinsmolecular motornucleoprotein packaging complexesterminasevirus assembly

Identifiers

PMID31473160
PMCPMC7416571

What Socratic holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.