ArticleScientific reports2019
A novel metabarcoding diagnostic tool to explore protozoan haemoparasite diversity in mammals: a proof-of-concept study using canines from the tropics.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed.
- From Field to Lab: Giant Toad (Rhinella horribilis) as an Emerging Model for the Study of Amphibian Blood Parasites.Integrative zoology · 2026Article
- Enhanced blood parasite species identification using V4-V9 18S rDNA barcoding by universal primers on a nanopore platform.Scientific reports · 2025Article
- Genetic characterization of zoonoticMicrobiology spectrum · 2025Article
- Individual variation within parasite communities of endangered African lions.Royal Society open science · 2025Article
- Characterizing the Helminth Community of the Mountain Gazelle (Gazella gazella Pallas, 1766) Through DNA Metabarcoding.Acta parasitologica · 2025Article
- Anaplasmosis in the Amazon: diagnostic challenges, persistence, and control ofFrontiers in veterinary science · 2025Review
- Molecular Detection of Kinetoplastid Species in Ticks and Fleas Associated with Dogs and Humans in Mexico.Pathogens (Basel, Switzerland) · 2024Article
- Development and validation of a long-read metabarcoding platform for the detection of filarial worm pathogens of animals and humans.BMC microbiology · 2024Article
- Next-generation sequencing metabarcoding assays reveal diverse bacterial vector-borne pathogens of Mongolian dogs.Current research in parasitology & vector-borne diseases · 2024Article
- Dogs are reservoir hosts of the zoonoticOne health (Amsterdam, Netherlands) · 2023Article
- Bovine Piroplasma Populations in the Philippines Characterized Using Targeted Amplicon Deep Sequencing.Microorganisms · 2023Article
- Nanopore Sequencing Using the Full-Length 16S rRNA Gene for Detection of Blood-Borne Bacteria in Dogs Reveals a Novel Species of Hemotropic Mycoplasma.Microbiology spectrum · 2022Article
- High-throughput microfluidic real-time PCR for the simultaneous detection of selected vector-borne pathogens in dogs in Bosnia and Herzegovina.Transboundary and emerging diseases · 2022Article
- Alveolates (dinoflagellates, ciliates and apicomplexans) and Rhizarians are the most common microbial eukaryotes in temperate Appalachian karst caves.Environmental microbiology reports · 2022Article
- Trypanosomatid Richness Among Rats, Opossums, and Dogs in the Caatinga Biome, Northeast Brazil, a Former Endemic Area of Chagas Disease.Frontiers in cellular and infection microbiology · 2022Article
- Field trial investigating the efficacy of a long-acting imidacloprid 10%/flumethrin 4.5% polymer matrix collar (Seresto®, Elanco) compared to monthly topical fipronil for the chemoprevention of canine tick-borne pathogens in Cambodia.Current research in parasitology & vector-borne diseases · 2022Article
- Infectious disease macroecology: parasite diversity and dynamics across the globe.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2021Article
- Data analysis workflow for the detection of canine vector-borne pathogens using 16 S rRNA Next-Generation Sequencing.BMC veterinary research · 2021Article
- Novel High-Throughput Multiplex qPCRs for the Detection of Canine Vector-Borne Pathogens in the Asia-Pacific.Microorganisms · 2021Article
- Exploring Prokaryotic and Eukaryotic Microbiomes Helps in Detecting Tick-Borne Infectious Agents in the Blood of Camels.Pathogens (Basel, Switzerland) · 2021Article
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Haemoparasites are responsible for some of the most prevalent and debilitating canine illnesses across the globe, whilst also posing a significant zoonotic risk to humankind. Nowhere are the effects of such parasites more pronounced than in developing countries in the tropics where the abundance and diversity of ectoparasites that transmit these pathogens reaches its zenith. Here we describe the use of a novel next-generation sequencing (NGS) metabarcoding based approach to screen for a range of blood-borne apicomplexan and kinetoplastid parasites from populations of temple dogs in Bangkok, Thailand. Our methodology elucidated high rates of Hepatozoon canis and Babesia vogeli infection, whilst also being able to characterise co-infections. In addition, our approach was confirmed to be more sensitive than conventional endpoint PCR diagnostic methods. Two kinetoplastid infections were also detected, including one by Trypanosoma evansi, a pathogen that is rarely screened for in dogs and another by Parabodo caudatus, a poorly documented organism that has been previously reported inhabiting the urinary tract of a dog with haematuria. Such results demonstrate the power of NGS methodologies to unearth rare and unusual pathogens, especially in regions of the world where limited information on canine vector-borne haemoparasites exist.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.