Evidence map›Paper›PMID 31497752›Full record

ArticleNature metabolism2019

Dynamic interactions of ABHD5 with PNPLA3 regulate triacylglycerol metabolism in brown adipocytes.

Alexander Yang, Emilio P Mottillo, Ljiljana Mladenovic-Lucas, Li Zhou, James G Granneman

Abstract read
In one paragraph

Article in Nature metabolism, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 65 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
65citing papers in PubMed, 1 pooled it
7.7field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

65 citing papers in PubMed, 1 synthesis or guideline pooled it, 115 citations in OpenAlex.

  1. Pooled it
  2. Autophagy in MASLD: A Metabolic and Precision Medicine Perspective.Liver international : official journal of the International Association for the Study of the Liver · 2026
    Review
  3. Article
  4. Haematologica · 2026
    Article
  5. Article
  6. Caspase-6 Controls Lipid and Energy Metabolism in Diet-Induced Obesity.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026
    Article
  7. Review
  8. Article
  9. Article
  10. Article
  11. Gene-based therapies for steatotic liver disease.Molecular therapy : the journal of the American Society of Gene Therapy · 2025
    Review
  12. Essential Biology of Lipid Droplets.Annual review of biochemistry · 2025
    Review
  13. Article
  14. Experimental Models to Investigate PNPLA3 in Liver Steatosis.Liver international : official journal of the International Association for the Study of the Liver · 2025
    Review
  15. Article
  16. Review
  17. Review
  18. Review
  19. Article
  20. Genetics of Metabolic Dysfunction-associated Steatotic Liver Disease: The State of the Art Update.Clinical gastroenterology and hepatology : the official clinical practice journal of the American Gastroenterological Association · 2024
    Review

5 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Alexander YangCenter for Molecular Medicine and Genetics, Wayne State University School of Medicine, Detroit, MI, USA 48201.
Emilio P MottilloCenter for Molecular Medicine and Genetics, Wayne State University School of Medicine, Detroit, MI, USA 48201.
Ljiljana Mladenovic-LucasCenter for Molecular Medicine and Genetics, Wayne State University School of Medicine, Detroit, MI, USA 48201.
Li ZhouCenter for Molecular Medicine and Genetics, Wayne State University School of Medicine, Detroit, MI, USA 48201.
James G GrannemanCenter for Molecular Medicine and Genetics, Wayne State University School of Medicine, Detroit, MI, USA 48201.
Wayne State University · US

Funding

Tumor Biology and Microenvironment (Program 1)P30CA022453 · NCI · WAYNE STATE UNIVERSITY · PI Jennifer L Beebe-Dimmer · 1985 to 2026
$68.4M
Regional Pilot And Feasibility Study Grants ProgramP30DK020572 · NIDDK · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI DAVID P OLSON · 2013 to 2026
$24.3M
Analysis of Cellular Plasticity in White Adipose Tissue.R01DK062292 · NIDDK · WAYNE STATE UNIVERSITY · PI James G Granneman · 2003 to 2026
$7.6M
Analysis of Lipolytic Trafficking in Adipocytes.R01DK076629 · NIDDK · WAYNE STATE UNIVERSITY · PI James G Granneman, Christopher V Kelly · 2009 to 2026
$7.1M
Preclinical validation of ABHD5 as a target for treatment of obesity.R01DK105963 · NIDDK · WAYNE STATE UNIVERSITY · PI GRANNEMAN, JAMES G · 2015 to 2018
$2.7M
Direct analysis of lipolysis-mediated signaling eventsR00DK114471 · NIDDK · WAYNE STATE UNIVERSITY · PI MOTTILLO, EMILIO · 2019 to 2021
$747k
Triple Quadrupole - Ion Trap Hybrid LC/MS/MS SystemS10RR027926 · NCRR · WAYNE STATE UNIVERSITY · PI MADDIPATI, KRISHNARAO · 2010 to 2010
$425k
Direct analysis of lipolysis-mediated signalingK99DK114471 · NIDDK · WAYNE STATE UNIVERSITY · PI MOTTILLO, EMILIO · 2017 to 2018
$178k
ABHD5 and PNPLA3 in the development of fatty liver diseaseF30DK116529 · NIDDK · WAYNE STATE UNIVERSITY · PI YANG, ALEXANDER C · 2018 to 2021
$168k
NCI NIH HHS P30 CA022453NCRR NIH HHS S10 RR027926NIDDK NIH HHS F30 DK116529NIDDK NIH HHS K99 DK114471NIDDK NIH HHS P30 DK020572NIDDK NIH HHS R00 DK114471NIDDK NIH HHS R01 DK062292NIDDK NIH HHS R01 DK076629NIDDK NIH HHS R01 DK105963
6 · The paper itself

Abstract

Patatin-Like Phospholipase Domain Containing 2 (PNPLA2)/Adipose Triglyceride Lipase (ATGL) and PNPLA3/Adiponutrin are close paralogs that appear to have opposite functions on triacylglycerol (TAG) mobilization and storage. PNPLA2/ATGL is a major triglyceride lipase in adipose tissue and liver, whereas a common human variant of PNPLA3, I148M, greatly increases risk of hepatosteatosis. Nonetheless, the function of PNPLA3 and the mechanism by which the I148M variant promotes TAG accumulation are poorly understood. Here we demonstrate that PNPLA3 strongly interacts with α/β hydrolase domain-containing 5 (ABHD5/CGI-58), an essential co-activator of PNPLA2/ATGL. Molecular imaging experiments demonstrate that PNPLA3 effectively competes with PNPLA2/ATGL for ABHD5, and that PNPLA3 I148M is more effective in this regard. Inducible overexpression of PNPLA3 I148M greatly suppressed PNPLA2/ATGL-dependent lipolysis and triggered massive TAG accumulation in brown adipocytes, and these effects were dependent on ABHD5. The interaction of PNPLA3 and ABHD5 can be regulated by fatty acid supplementation and synthetic ABHD5 ligands, raising the possibility that this interaction might be targeted for treatment of fatty liver disease.

Indexed as

1-Acylglycerol-3-Phosphate O-AcyltransferaseAcyltransferasesAdipocytes, BrownCells, CulturedEndoplasmic ReticulumFatty AcidsHumansLigandsLipaseMembrane ProteinsPhospholipases A2, Calcium-IndependentProtein BindingProtein TransportTriglycerides1-Acylglycerol-3-Phosphate O-AcyltransferaseABHD5 protein, humanAcyltransferasesFatty AcidsLigandsLipaseMembrane ProteinsPhospholipases A2, Calcium-IndependentPNPLA3 protein, humanTriglycerides

Identifiers

PMID31497752
PMCPMC6730670
OpenAlexW2943689056

What Socratic holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.