Evidence map›Paper›PMID 31506750›Full record

ArticleInternational journal of clinical oncology2020

Exosomal miR-1246 in serum as a potential biomarker for early diagnosis of gastric cancer.

Yuntao Shi, Zhonghong Wang, Xiaojuan Zhu, Ling Chen, Yilan Ma, Jiayan Wang, Xiaozhong Yang, Zheng Liu

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In one paragraph

Article in International journal of clinical oncology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 82 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
82citing papers in PubMed, 2 pooled it
5.0field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

82 citing papers in PubMed, 2 syntheses or guidelines pooled it, 121 citations in OpenAlex.

  1. Pooled it
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  6. Diagnostic and prognostic significance of circulating extracellular vesicles in gastrointestinal cancers.Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico · 2026
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  18. Tumor-exosomal miR-205-5p as a diagnostic biomarker for colorectal cancer.Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico · 2025
    Article
  19. Article
  20. Review

22 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 1 country.

Yuntao ShiMedical Center for Digestive Diseases, The Second Affiliated Hospital of Nanjing Medical University, No.121, Jiangjiayuan Road, Nanjing, 210011, China.
Zhonghong WangDepartment of Gastroenterology, Huaian People's Hospital of Hongze District, Huaian, 223100, China.
Xiaojuan ZhuMedical Center for Digestive Diseases, The Second Affiliated Hospital of Nanjing Medical University, No.121, Jiangjiayuan Road, Nanjing, 210011, China.
Ling ChenMedical Center for Digestive Diseases, The Second Affiliated Hospital of Nanjing Medical University, No.121, Jiangjiayuan Road, Nanjing, 210011, China.
Yilan MaMedical Center for Digestive Diseases, The Second Affiliated Hospital of Nanjing Medical University, No.121, Jiangjiayuan Road, Nanjing, 210011, China.
Jiayan WangMedical Center for Digestive Diseases, The Second Affiliated Hospital of Nanjing Medical University, No.121, Jiangjiayuan Road, Nanjing, 210011, China.
Xiaozhong YangDepartment of Gastroenterology, The Affiliated Huaian No. 1 People's Hospital of Nanjing Medical University, No.1, Huanghe West Road, Huaian, 223300, China. xz_yang1023@aliyun.com.
Zheng LiuMedical Center for Digestive Diseases, The Second Affiliated Hospital of Nanjing Medical University, No.121, Jiangjiayuan Road, Nanjing, 210011, China. liuzhenghjk@163.com.
Second Affiliated Hospital of Nanjing Medical University · CNHuaian First People’s Hospital · CNNanjing Medical University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundGastric cancer (GC) patients are usually diagnosed in advanced stages which results in high mortality. This study aimed to identify novel circulating miRNAs as biomarkers for the early detection of GC.

methodsCandidate miRNA was identified after integrated analysis of two Gene Expression Omnibus (GEO) datasets and clinical serum samples. Exosomes extracted were verified using transmission electron microscopy (TEM) and western blot. The expressions of miRNAs were tested through qRT-PCR. Receiver operating characteristic curve (ROC) analysis was used to explore the diagnostic utility of miRNAs. RNA pull-down assay was used to find RNA binding proteins (RBPs) which transport candidate miRNA into exosomes. Bioinformatics analysis of candidate miRNA was conducted using DAVID and Cytoscape.

resultsAfter integrated analysis of two GEO datasets, six circulating miRNAs were found to be consistently upregulated in GC patients. Then, qRT-PCR demonstrated that serum miR-1246 was the one with the largest fold change. Studies in vitro revealed that elevated serum miR-1246 was tumor-derived by being packaged into exosomes with the help of ELAVL1. Thereafter, we discovered that exosomal miR-1246 expressions in serum could differentiate GC patients with TNM stage I from healthy controls (HCs) and patients with benign diseases (BDs) with area under the curve (AUC) of 0.843 and 0.811, respectively. Bioinformatics analysis revealed miR-1246, as a tumor suppressor in GC, could regulate several signaling pathways.

conclusionCirculating exosomal miR-1246 was a potential biomarker for the early diagnosis of GC.

Indexed as

AdultBiomarkers, TumorEarly Detection of CancerELAV-Like Protein 1ExosomesFemaleHumansMaleMicroRNAsMiddle AgedROC CurveStomach NeoplasmsBiomarkers, TumorELAVL1 protein, humanELAV-Like Protein 1MicroRNAsMIRN1246 microRNA, humanBiomarkerDiagnosisExosomesGastric cancermiR-1246

Identifiers

PMID31506750
OpenAlexW2973031927

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.