ArticleMolecular biotechnology2019
Expression of Drosera rotundifolia Chitinase in Transgenic Tobacco Plants Enhanced Their Antifungal Potential.
Article in Molecular biotechnology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 29 citations in OpenAlex.
- Genome-Wide Analysis Reveals Chitinases as Putative Defense-Related Proteins Against Fungi in the Genomes ofPlants (Basel, Switzerland) · 2025Article
- Nepenthes chitinase NkChit2b- 1 confers broad-spectrum resistance to chitin-containing pathogens and insects in plants.Advanced biotechnology · 2025Article
- Characterization of the chitinase gene family in Saccharum reveals the disease resistance mechanism of ScChiVII1.Plant cell reports · 2024Article
- Characterization of soybean chitinase genes induced by rhizobacteria involved in the defense againstFrontiers in plant science · 2024Article
- Article
- Overexpression of flowering locus D (FLD) in Indian mustard (Brassica juncea) enhances tolerance to Alternaria brassicae and Sclerotinia sclerotiorum.Plant cell reports · 2023Article
- Article
- Basic β-1,3-Glucanase fromPlants (Basel, Switzerland) · 2021Article
- Genome-Wide Identification and Expression of Chitinase Class I Genes in Garlic (Plants (Basel, Switzerland) · 2021Article
- Salicylate-Induced Chitinases in Pea Roots.Doklady. Biochemistry and biophysics · 2020Article
- Inhibitory Mechanism ofPlants (Basel, Switzerland) · 2020Article
- Morphological Responses and Gene Expression of Grain Amaranth (Plants (Basel, Switzerland) · 2020Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
In this study, a chitinase gene (DrChit) that plays a role in the carnivorous processes of Drosera rotundifolia L. was isolated from genomic DNA, linked to a double CaMV35S promoter and nos terminator in a pBinPlus plant binary vector, and used for Agrobacterium-mediated transformation of tobacco. RT-qPCR revealed that within 14 transgenic lines analysed in detail, 57% had DrChit transcript abundance comparable to or lower than level of a reference actin gene transcript. In contrast, the transgenic lines 9 and 14 exhibited 72 and 152 times higher expression level than actin. The protein extracts of these two lines exhibited five and eight times higher chitinolytic activity than non-transgenic controls when measured in a fluorimetric assay with FITC-chitin. Finally, the growth of Trichoderma viride was obviously suppressed when the pathogen was exposed to 100 μg of crude protein extract isolated from line 9 and line 14, with the area of mycelium growth reaching only 56.4% and 45.2%, of non-transgenic control, respectively. This is the first time a chitinase from a carnivorous plant with substrate specificity for long chitin polymers was tested in a transgenic plant with the aim of exploring its antifungal potential.
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