Evidence map›Paper›PMID 31562379›Full record

ArticleScientific reports2019

PKN1 kinase-negative knock-in mice develop splenomegaly and leukopenia at advanced age without obvious autoimmune-like phenotypes.

Salman Mahmud Siddique, Koji Kubouchi, Yuka Shinmichi, Nana Sawada, Reiko Sugiura, Yasushi Itoh, Shunsuke Uehara, Kanae Nishimura, Shunsuke Okamura, Hiroyuki Ohsaki and 7 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.5field-weighted citation impact, top 26% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 5 citations in OpenAlex.

  1. Article
  2. Review
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

17 authors at 7 institutions in 1 country.

Salman Mahmud SiddiqueGraduate School of Medicine, Kobe University, Kobe, 650-0017, Japan.
Koji KubouchiGraduate School of Medicine, Kobe University, Kobe, 650-0017, Japan.
Yuka ShinmichiLaboratory of Molecular Pharmacogenomics, School of Pharmaceutical Sciences, Kindai University, 3-4-1, Kowakae, Higashi-Osaka, 577- 8502, Japan.
Nana SawadaLaboratory of Molecular Pharmacogenomics, School of Pharmaceutical Sciences, Kindai University, 3-4-1, Kowakae, Higashi-Osaka, 577- 8502, Japan.
Reiko SugiuraLaboratory of Molecular Pharmacogenomics, School of Pharmaceutical Sciences, Kindai University, 3-4-1, Kowakae, Higashi-Osaka, 577- 8502, Japan.
Yasushi ItohDepartment of Pathology, Shiga University of Medical Science, Otsu, Shiga, Japan.
Shunsuke UeharaDepartment of Biochemistry, Matsumoto Dental University, Shiojiri, Nagano, 399-0781, Japan.
Kanae NishimuraLaboratory of Pathology, Department of Medical Biophysics, Kobe University Graduate School of Health Sciences, 7-10-2 Tomogaoka, Suma, Kobe, Hyogo, 654-0142, Japan.
Shunsuke OkamuraLaboratory of Pathology, Department of Medical Biophysics, Kobe University Graduate School of Health Sciences, 7-10-2 Tomogaoka, Suma, Kobe, Hyogo, 654-0142, Japan.
Hiroyuki OhsakiLaboratory of Pathology, Department of Medical Biophysics, Kobe University Graduate School of Health Sciences, 7-10-2 Tomogaoka, Suma, Kobe, Hyogo, 654-0142, Japan.ORCID http://orcid.org/0000-0001-6046-3785
Shingo KamoshidaLaboratory of Pathology, Department of Medical Biophysics, Kobe University Graduate School of Health Sciences, 7-10-2 Tomogaoka, Suma, Kobe, Hyogo, 654-0142, Japan.
Yusuke YamashitaDepartment of Hematology/Oncology, Wakayama Medical University, Wakayama, Japan.
Shinobu TamuraDepartment of Hematology/Oncology, Wakayama Medical University, Wakayama, Japan.
Takashi SonokiDepartment of Hematology/Oncology, Wakayama Medical University, Wakayama, Japan.
Hiroshi MatsuokaDivision of Medical Oncology and Hematology, Kobe University Hospital, Kobe, Hyogo, Japan.
Tomoo ItohDepartment of Diagnostic Pathology, Kobe University Hospital, Kobe, Hyogo, 650-0017, Japan.
Hideyuki MukaiGraduate School of Medicine, Kobe University, Kobe, 650-0017, Japan. mukinase@kobe-u.ac.jp.
Kobe University · JPKindai University · JPWakayama Medical University · JPKobe University Hospital · JPKobe Pharmaceutical University · JPMatsumoto Dental University · JPShiga University of Medical Science · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Protein kinase N1 (PKN1) knockout (KO) mice spontaneously form germinal centers (GCs) and develop an autoimmune-like disease with age. Here, we investigated the function of PKN1 kinase activity in vivo using aged mice deficient in kinase activity resulting from the introduction of a point mutation (T778A) in the activation loop of the enzyme. PKN1[T778A] mice reached adulthood without external abnormalities; however, the average spleen size and weight of aged PKN1[T778A] mice increased significantly compared to aged wild type (WT) mice. Histologic examination and Southern blot analyses of spleens showed extramedullary hematopoiesis and/or lymphomagenesis in some cases, although without significantly different incidences between PKN1[T778A] and WT mice. Additionally, flow cytometry revealed increased numbers in B220

Indexed as

Age FactorsAnimalsGene Knock-In TechniquesGerminal CenterLeukopeniaMiceMice, KnockoutPhosphorylationProtein Kinase CSignal TransductionSplenomegalyProtein Kinase Cprotein kinase N

Identifiers

PMID31562379
PMCPMC6764976
OpenAlexW2976666854

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.