ArticleJournal of neurochemistry2020
Heterogeneous glutamatergic receptor mRNA expression across phrenic motor neurons in rats.
Article in Journal of neurochemistry, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 37 citations in OpenAlex.
- Pairing ampakine with brief hypoxia evokes phrenic neuroplasticity via a spinal NMDA receptor-mediated mechanism.Function (Oxford, England) · 2026Article
- Motor Neuron Size-Dependent Differences in mRNA Expression of Tropomyosin-Related Kinase Receptor B in Adult Rats.Journal of neurochemistry · 2026Article
- An update on spinal cord injury and diaphragm neuromotor control.Expert review of respiratory medicine · 2025Review
- Article
- Pharmacological modulation of respiratory control: Ampakines as a therapeutic strategy.Pharmacology & therapeutics · 2025Review
- PTEN inhibition promotes robust growth of bulbospinal respiratory axons and partial recovery of diaphragm function in a chronic model of cervical contusion spinal cord injury.Experimental neurology · 2024Article
- Ampakines increase diaphragm activation following mid-cervical contusion injury in rats.Experimental neurology · 2024Article
- Article
- Pattern sensitivity of ampakine-hypoxia interactions for evoking phrenic motor facilitation in anesthetized rat.Journal of neurophysiology · 2024Article
- Acute ampakines increase voiding function and coordination in a rat model of SCI.bioRxiv : the preprint server for biology · 2023Article
- Novel regenerative drug, SPG302 promotes functional recovery of diaphragm muscle activity after cervical spinal cord injury.The Journal of physiology · 2023Article
- Aging affects the number and morphological heterogeneity of rat phrenic motor neurons and phrenic motor axons.Physiological reports · 2023Article
- Targeting drug or gene delivery to the phrenic motoneuron pool.Journal of neurophysiology · 2023Review
- SCAMPR, a single-cell automated multiplex pipeline for RNA quantification and spatial mapping.Cell reports methods · 2022Article
- Cervical spinal hemisection alters phrenic motor neuron glutamatergic mRNA receptor expression.Experimental neurology · 2022Article
- Spinally delivered ampakine CX717 increases phrenic motor output in adult rats.Respiratory physiology & neurobiology · 2022Article
- The phrenic neuromuscular system.Handbook of clinical neurology · 2022Review
- Ampakines Stimulate Diaphragm Activity after Spinal Cord Injury.Journal of neurotrauma · 2021Article
- Acute intrathecal BDNF enhances functional recovery after cervical spinal cord injury in rats.Journal of neurophysiology · 2021Article
- Quantifying mitochondrial volume density in phrenic motor neurons.Journal of neuroscience methods · 2021Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The diaphragm muscle comprises various types of motor units that are recruited in an orderly fashion governed by the intrinsic electrophysiological properties (membrane capacitance as a function of somal surface area) of phrenic motor neurons (PhMNs). Glutamate is the main excitatory neurotransmitter at PhMNs and acts primarily via fast acting AMPA and N-methyl-D-aspartic acid (NMDA) receptors. Differences in receptor expression may also contribute to motor unit recruitment order. We used single cell, multiplex fluorescence in situ hybridization to determine glutamatergic receptor mRNA expression across PhMNs based on their somal surface area. In adult male and female rats (n = 9) PhMNs were retrogradely labeled for analyses (n = 453 neurons). Differences in the total number and density of mRNA transcripts were evident across PhMNs grouped into tertiles according to somal surface area. A ~ 25% higher density of AMPA (Gria2) and NMDA (Grin1) mRNA expression was evident in PhMNs in the lower tertile compared to the upper tertile. These smaller PhMNs likely comprise type S motor units that are recruited first to accomplish lower force, ventilatory behaviors. In contrast, larger PhMNs with lower volume densities of AMPA and NMDA mRNA expression presumably comprise type FInt and FF motor units that are recruited during higher force, expulsive behaviors. Furthermore, there was a significantly higher cytosolic NMDA mRNA expression in small PhMNs suggesting a more important role for NMDA-mediated glutamatergic neurotransmission at smaller PhMNs. These results are consistent with the observed order of motor unit recruitment and suggest a role for glutamatergic receptors in support of this orderly recruitment. Cover Image for this issue: doi: 10.1111/jnc.14747.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.