ArticleNature communications2019
A tunable dual-input system for on-demand dynamic gene expression regulation.
Article in Nature communications, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
23 citing papers in PubMed.
- EGFRvIII Reduces Neural Stem/Progenitor Marker Expression in GFAP-Negative iNSCs: Evidence for a Context-Dependent Cellular Response.International journal of molecular sciences · 2026Article
- Development and Application of a Cumate-Inducible Promoter, PMicrobial biotechnology · 2026Article
- A Novel Single-Layer Microfluidic Device for Dynamic Stimulation, Culture, and Imaging of Mammalian Cells.Biosensors · 2025Article
- A simplified two-plasmid system for orthogonal control of mammalian gene expression using light-activated CRISPR effector.BMC biotechnology · 2025Article
- Small molecule- and cell contact-inducible systems for controlling expression and differentiation in mouse embryonic stem cells.Development (Cambridge, England) · 2025Article
- Efficient gene delivery admitted by small metabolites specifically targeting astrocytes in the mouse brain.Molecular therapy : the journal of the American Society of Gene Therapy · 2025Article
- Cell factory engineering: Challenges and opportunities for synthetic biology applications.Biotechnology and bioengineering · 2023Review
- In-silico and in-vitro morphometric analysis of intestinal organoids.PLoS computational biology · 2023Article
- Segmentation strategy of de novo designed four-helical bundles expands protein oligomerization modalities for cell regulation.Nature communications · 2023Article
- Titrating Avidity of Yeast-Displayed Proteins Using a Transcriptional Regulator.ACS synthetic biology · 2023Article
- An optimized Tet-On system for conditional control of gene expression in sea urchins.Development (Cambridge, England) · 2023Article
- Control-Based Continuation: A New Approach to Prototype Synthetic Gene Networks.ACS synthetic biology · 2022Article
- Integrating transcription-factor abundance with chromatin accessibility in human erythroid lineage commitment.Cell reports methods · 2022Article
- β-catenin perturbations control differentiation programs in mouse embryonic stem cells.iScience · 2022Article
- Protease-controlled secretion and display of intercellular signals.Nature communications · 2022Article
- The context matrix: Navigating biological complexity for advanced biodesign.Frontiers in bioengineering and biotechnology · 2022Article
- Pulsed Electric Fields Alter Expression of NF-κB Promoter-Controlled Gene.International journal of molecular sciences · 2021Article
- ChipSeg: An Automatic Tool to Segment Bacterial and Mammalian Cells Cultured in Microfluidic Devices.ACS omega · 2021Article
- Orthogonal control of mean and variability of endogenous genes in a human cell line.Nature communications · 2021Article
- A Microfluidic/Microscopy-Based Platform for on-Chip Controlled Gene Expression in Mammalian Cells.Methods in molecular biology (Clifton, N.J.) · 2021Article
Corrections and comments
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Authors and funding
9 authors.
Funding
Abstract
Cellular systems have evolved numerous mechanisms to adapt to environmental stimuli, underpinned by dynamic patterns of gene expression. In addition to gene transcription regulation, modulation of protein levels, dynamics and localization are essential checkpoints governing cell functions. The introduction of inducible promoters has allowed gene expression control using orthogonal molecules, facilitating its rapid and reversible manipulation to study gene function. However, differing protein stabilities hinder the generation of protein temporal profiles seen in vivo. Here, we improve the Tet-On system integrating conditional destabilising elements at the post-translational level and permitting simultaneous control of gene expression and protein stability. We show, in mammalian cells, that adding protein stability control allows faster response times, fully tunable and enhanced dynamic range, and improved in silico feedback control of gene expression. Finally, we highlight the effectiveness of our dual-input system to modulate levels of signalling pathway components in mouse Embryonic Stem Cells.
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What Socratic holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.