Evidence map›Paper›PMID 31600142›Full record

ArticleAging2019

LncRNA TTN-AS1 regulates osteosarcoma cell apoptosis and drug resistance via the miR-134-5p/MBTD1 axis.

Dong Fu, Chunwen Lu, Xingzhou Qu, Peng Li, Kai Chen, Liancheng Shan, Xiaodong Zhu

Open access · greenAbstract read
In one paragraph

Article in Aging, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 85 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
85citing papers in PubMed, 1 pooled it
9.7field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

85 citing papers in PubMed, 1 synthesis or guideline pooled it, 155 citations in OpenAlex.

  1. Pooled it
  2. Article
  3. Article
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  5. Article
  6. Article
  7. Article
  8. Osteosarcoma in a ceRNET perspective.Journal of biomedical science · 2024
    Review
  9. Review
  10. Article
  11. Emerging roles of long non-coding RNAs in osteosarcoma.Frontiers in molecular biosciences · 2024
    Review
  12. Article
  13. Article
  14. Article
  15. Article
  16. Review
  17. Article
  18. Article
  19. Article
  20. CircZNF236 facilitates malignant progression in oral squamous cell carcinoma by sequestering miR-145-5p.Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico · 2023
    Article

25 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 4 institutions in 1 country.

Dong FuChildren's Hospital of Shanghai, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.
Chunwen LuDepartment of Spinal Surgery, Changhai Hospital, Affiliated to Naval Military Medical University, Shanghai, China.
Xingzhou QuThe Department of Oral Maxillofacial and Head Neck Oncology in the Ninth Hospital Affiliated Shanghai Jiaotong University, Shanghai, China.
Peng LiChildren's Hospital of Shanghai, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.
Kai ChenHai'an People's Hospital, Hai'an, Nantong, Jiangsu, China.
Liancheng ShanDepartment of Orthopedics, Tongren Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Xiaodong ZhuDepartment of Orthopedics, Tongren Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Shanghai Jiao Tong University · CNChanghai Hospital · CNShanghai Children's Hospital · CNShanghai Ninth People's Hospital · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

aimTo explore the mechanism by which long non-coding RNA (lncRNA) TTN-AS1 regulates osteosarcoma cell apoptosis and drug resistance via the microRNA miR-134-5p/malignant brain tumour domain containing 1 (MBTD1) axis.

resultsThe lncRNA TTN-AS1 was highly expressed in osteosarcoma and was associated with poor prognosis. The lncRNA TTN-AS1 promoted cell viability and inhibited apoptosis. MiR-134-5p targeted MBTD1, which was regulated by lncRNA TTN-AS1. MBTD1 was highly expressed in osteosarcoma and was associated with poor prognosis. MBTD1 promoted cell viability and inhibited apoptosis, and knockdown of MBTD1 reversed the cancer-promoting effects of lncRNA TTN-AS1. Downregulation of lncRNA TTN-AS1 reduced drug resistance.

conclusionIn osteosarcoma, lncRNA TTN-AS1 promoted the expression of MBTD1 by targeting miR-134-5p and regulated cell growth, apoptosis and drug resistance.

methodsThe expression characteristics of genes in osteosarcoma patients were analysed using bioinformatics. Plasmid transfection technology was applied to silence or overexpress lncRNA TTN-AS1, miR-134-5p and MBTD1. Western blotting and quantitative polymerase chain reaction (qPCR) were used to detect protein and RNA, respectively. A cell counting kit 8 (CCK-8) and flow cytometry were used to detect cell viability and apoptosis. The effects of lncRNA TTN-AS1 and MBTD1 on osteosarcoma in vivo were studied by using a tumour burden assay.

Indexed as

ApoptosisOsteosarcomaAgingBrain NeoplasmsCell Line, TumorCell ProliferationChromosomal Proteins, Non-HistoneComputational BiologyDrug ResistanceGene Expression Regulation, NeoplasticHumansMicroRNAsRNA, Long NoncodingChromosomal Proteins, Non-HistoneMBTD1 protein, humanMicroRNAsMIRN134 microRNA, humanRNA, Long Noncodingaging and age-related diseaseslncRNA TTN-AS1malignant brain tumour domain containing protein 1miR-134-5posteosarcomaresistance

Identifiers

PMID31600142
PMCPMC6814585
OpenAlexW2980145189

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.