Evidence mapPaperPMID 31603345Full record

ArticleAmerican journal of physiology. Heart and circulatory physiology2019

Persistent insulin signaling coupled with restricted PI3K activation causes insulin-induced vasoconstriction.

T Dylan Olver, Zachary I Grunewald, Thaysa Ghiarone, Robert M Restaino, Allan R K Sales, Lauren K Park, Pamela K Thorne, Rama Rao Ganga, Craig A Emter, Peter W R Lemon and 4 more

Open access · greenAbstract read
In one paragraph

Article in American journal of physiology. Heart and circulatory physiology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.

0numbers the graph read from it
0cells of the map it votes in
18citing papers in PubMed
1.7field-weighted citation impact, top 15% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

18 citing papers in PubMed, 22 citations in OpenAlex.

  1. Trial
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  5. The metabolically protective energy expenditure increase ofAmerican journal of physiology. Endocrinology and metabolism · 2025
    Article
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  8. Impact of sex and diet-induced weight loss on vascular insulin sensitivity in type 2 diabetes.American journal of physiology. Regulatory, integrative and comparative physiology · 2023
    Article
  9. New insights into mechanisms of endothelial insulin resistance in type 2 diabetes.American journal of physiology. Heart and circulatory physiology · 2022
    Review
  10. Article
  11. Review
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  15. Review
  16. Endothelial dysfunction due to selective insulin resistance in vascular endothelium: insights from mechanistic modeling.American journal of physiology. Endocrinology and metabolism · 2020 · on this map
    Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors at 4 institutions in 3 countries.

T Dylan OlverDepartment of Biomedical Sciences, Western College of Veterinary Medicine, University of Saskatchewan, Saskatchewan, Canada.
Zachary I GrunewaldDepartment of Nutrition and Exercise Physiology, University of Missouri, Columbia, Missouri.
Thaysa GhiaroneDalton Cardiovascular Research Center, University of Missouri, Columbia, Missouri.
Robert M RestainoDalton Cardiovascular Research Center, University of Missouri, Columbia, Missouri.
Allan R K SalesDalton Cardiovascular Research Center, University of Missouri, Columbia, Missouri.
Lauren K ParkDepartment of Nutrition and Exercise Physiology, University of Missouri, Columbia, Missouri.
Pamela K ThorneDepartment of Biomedical Sciences, University of Missouri, Columbia, Missouri.
Rama Rao GangaDepartment of Surgery, University of Missouri, Columbia, Missouri.
Craig A EmterDepartment of Biomedical Sciences, University of Missouri, Columbia, Missouri.
Peter W R LemonSchool of Kinesiology, The University of Western Ontario, London, Ontario, Canada.
J Kevin ShoemakerSchool of Kinesiology, The University of Western Ontario, London, Ontario, Canada.
Camila Manrique-AcevedoDalton Cardiovascular Research Center, University of Missouri, Columbia, Missouri.
Luis A Martinez-LemusDalton Cardiovascular Research Center, University of Missouri, Columbia, Missouri.
Jaume PadillaDepartment of Nutrition and Exercise Physiology, University of Missouri, Columbia, Missouri.
University of Missouri · USWestern University · CAD’Or Institute for Research and Education · BRUniversity of Saskatchewan · CA

Funding

NHLBI NIH HHS K01 HL125503NHLBI NIH HHS K08 HL129074NHLBI NIH HHS R01 HL088105NHLBI NIH HHS R01 HL112998NHLBI NIH HHS R01 HL137769
6 · The paper itself

Abstract

Insulin modulates vasomotor tone through vasodilator and vasoconstrictor signaling pathways. The purpose of the present work was to determine whether insulin-stimulated vasoconstriction is a pathophysiological phenomenon that can result from a combination of persistent insulin signaling, suppressed phosphatidylinositol-3 kinase (PI3K) activation, and an ensuing relative increase in MAPK/endothelin-1 (ET-1) activity. First, we examined previously published work from our group where we assessed changes in lower-limb blood flow in response to an oral glucose tolerance test (endogenous insulin stimulation) in lean and obese subjects. The new analyses showed that the peak rise in vascular resistance during the postprandial state was greater in obese compared with lean subjects. We next extended on these findings by demonstrating that insulin-induced vasoconstriction in isolated resistance arteries from obese subjects was attenuated with ET-1 receptor antagonism, thus implicating ET-1 signaling in this constriction response. Last, we examined in isolated resistance arteries from pigs the dual roles of persistent insulin signaling and blunted PI3K activation in modulating vasomotor responses to insulin. We found that prolonged insulin stimulation did not alter vasomotor responses to insulin when insulin-signaling pathways remained unrestricted. However, prolonged insulinization along with pharmacological suppression of PI3K activity resulted in insulin-induced vasoconstriction, rather than vasodilation. Notably, such aberrant vascular response was rescued with either MAPK inhibition or ET-1 receptor antagonism. In summary, we demonstrate that insulin-induced vasoconstriction is a pathophysiological phenomenon that can be recapitulated when sustained insulin signaling is coupled with depressed PI3K activation and the concomitant relative increase in MAPK/ET-1 activity.

Indexed as

AnimalsArteriesEndothelin-1Enzyme ActivationFemaleHumansInsulinInsulin ResistanceMaleMiddle AgedMitogen-Activated Protein KinasesObesityPhosphatidylinositol 3-KinaseSignal TransductionSus scrofaVasoconstrictionEndothelin-1InsulinMitogen-Activated Protein KinasesPhosphatidylinositol 3-Kinasediabetesendothelin-1MAPKobesityselective insulin resistance

Identifiers

PMID31603345
PMCPMC6879917
OpenAlexW2980227947

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.