ArticleProceedings of the National Academy of Sciences of the United States of America2019
Dynamics of trophoblast differentiation in peri-implantation-stage human embryos.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 62 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
62 citing papers in PubMed, 148 citations in OpenAlex.
- Trophoblast stem cells and syncytiotrophoblasts lack inflammatory responses to LPS but retain robust interferon-mediated antiviral immunity.Reproduction & fertility · 2026Article
- Single-cell transcriptomics reveals differences between chorionic and basal plate cytotrophoblasts and 2D-cultured trophoblast stem cells.Communications biology · 2026Article
- Variations in blood levels of the anti-fusogenic endogenous retroviral protein suppressyn are associated with fetal growth outcome in preeclampsia.Scientific reports · 2025Article
- Two classes of syncytiotrophoblast nuclei form following BAP-driven human pluripotent stem cell differentiation.Placenta · 2025Article
- Single Cell RNA Sequencing and Its Impact on Understanding Human Embryo Development.International journal of molecular sciences · 2025Review
- First trimester extravillous trophoblast secretes HLA class I molecules via small extracellular vesicles.Placenta · 2025Article
- Transient chemical-mediated epigenetic modulation confers unrestricted lineage potential on human primed pluripotent stem cells.Science China. Life sciences · 2025Article
- Development of apical out trophoblast stem cell derived organoids to model early human pregnancy.iScience · 2025Article
- The modeling of human implantation and early placentation: achievements and perspectives.Human reproduction update · 2025Review
- Sex differences in human pre-gastrulation embryos.Science China. Life sciences · 2025Article
- Proximate and ultimate causes of pregnancy sickness.Evolution, medicine, and public health · 2025Review
- Transcriptomic effects of alginate hydrogel applied to the production of bovine embryos.Heliyon · 2024Article
- The central role of creatine and polyamines in fetal growth restriction.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2024Article
- NImmunity, inflammation and disease · 2024Review
- In vitro culture of cynomolgus monkey embryos from blastocyst to early organogenesis.Nature protocols · 2024Review
- Organ-on-a-chip: future of female reproductive pathophysiological models.Journal of nanobiotechnology · 2024Review
- A single-cell transcriptome atlas of human euploid and aneuploid blastocysts.Nature genetics · 2024Article
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- Distinct pathways drive anterior hypoblast specification in the implanting human embryo.Nature cell biology · 2024Article
- Article
2 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors at 4 institutions in 1 country.
Funding
Abstract
Single-cell RNA sequencing of cells from cultured human blastocysts has enabled us to define the transcriptomic landscape of placental trophoblast (TB) that surrounds the epiblast and associated embryonic tissues during the enigmatic day 8 (D8) to D12 peri-implantation period before the villous placenta forms. We analyzed the transcriptomes of 3 early placental cell types, cytoTB (CTB), syncytioTB (STB), and migratoryTB (MTB), picked manually from cultured embryos dissociated with trypsin and were able to follow sublineages that emerged from proliferating CTB at the periphery of the conceptus. A unique form of CTB with some features of STB was detectable at D8, while mature STB was at its zenith at D10. A form of MTB with a mixed MTB/CTB phenotype arose around D10. By D12, STB generation was in decline, CTB had entered a new phase of proliferation, and mature MTB cells had begun to move from the main body of the conceptus. Notably, the MTB transcriptome at D12 indicated enrichment of transcripts associated with IFN signaling, migration, and invasion and up-regulation of HLA-C, HLA-E, and HLA-G. The STB, which is distinct from the STB of later villous STB, had a phenotype consistent with intense protein export and placental hormone production, as well as migration and invasion. The studies show that TB associated with human embryos is in rapid developmental flux during peri-implantation period when it must invade, signal robustly to the mother to ensure that the pregnancy continues, and make first contact with the maternal immune system.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.