Evidence map›Paper›PMID 31657719›Full record

ArticleeLife2019

Mechanisms of virus dissemination in bone marrow of HIV-1-infected humanized BLT mice.

Mark S Ladinsky, Wannisa Khamaikawin, Yujin Jung, Samantha Lin, Jennifer Lam, Dong Sung An, Pamela J Bjorkman, Collin Kieffer

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in eLife, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.

0numbers the graph read from it
0cells of the map it votes in
14citing papers in PubMed
3.6field-weighted citation impact, top 7% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

14 citing papers in PubMed, 30 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 4 institutions in 2 countries.

Mark S Ladinsky *Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, United States.
Wannisa Khamaikawin *School of Nursing, UCLA AIDS Institute, University of California, Los Angeles, Los Angeles, United States.
Yujin JungSchool of Nursing, UCLA AIDS Institute, University of California, Los Angeles, Los Angeles, United States.
Samantha LinSchool of Nursing, UCLA AIDS Institute, University of California, Los Angeles, Los Angeles, United States.
Jennifer LamSchool of Nursing, UCLA AIDS Institute, University of California, Los Angeles, Los Angeles, United States.
Dong Sung AnSchool of Nursing, UCLA AIDS Institute, University of California, Los Angeles, Los Angeles, United States.
Pamela J BjorkmanDivision of Biology and Biological Engineering, California Institute of Technology, Pasadena, United States.ORCID 0000-0002-2277-3990
Collin KiefferDivision of Biology and Biological Engineering, California Institute of Technology, Pasadena, United States.ORCID 0000-0001-9051-3819
California Institute of Technology · USUniversity of California, Los Angeles · USCedars-Sinai Medical Center · USJohns Hopkins University · US

Funding

X-ray Cryst. CoreP50GM082545 · NIGMS · UNIVERSITY OF UTAH · PI SUNDQUIST, WESLEY I. · 2007 to 2018
$51.1M
Virology CoreP30AI028697 · NIAID · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI KITCHEN, SCOTT G · 1991 to 2018
$39.9M
Virus TransmissionP50AI150464 · NIAID · UNIVERSITY OF UTAH · PI SUNDQUIST, WESLEY I. · 2019 to 2021
$14.9M
Genetic protection of hematopoietic stem cells for stable HIV controlR01AI100652 · NIAID · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI AN, DONG SUNG · 2012 to 2016
$1.9M
California HIV/AIDS Research Program ID15-CT-017California Institute of Technology Rosalind W. Alcott Postdoctoral FellowshipNational Institute of Allergy and Infectious Diseases 1R01AI100652-01A1National Institute of Allergy and Infectious Diseases AI028697NIAID NIH HHS P30 AI028697NIAID NIH HHS P50 AI150464NIAID NIH HHS R01 AI100652NIGMS NIH HHS 2 P50 GM082545-08NIGMS NIH HHS P50 GM082545University of Illinois at Urbana-Champaign School of Molecular and Cellular Biology Startup Funding
6 · The paper itself

Abstract

Immune progenitor cells differentiate in bone marrow (BM) and then migrate to tissues. HIV-1 infects multiple BM cell types, but virus dissemination within BM has been poorly understood. We used light microscopy and electron tomography to elucidate mechanisms of HIV-1 dissemination within BM of HIV-1-infected BM/liver/thymus (BLT) mice. Tissue clearing combined with confocal and light sheet fluorescence microscopy revealed distinct populations of HIV-1 p24-producing cells in BM early after infection, and quantification of these populations identified macrophages as the principal subset of virus-producing cells in BM over time. Electron tomography demonstrated three modes of HIV-1 dissemination in BM: (

Indexed as

AnimalsBone Marrow CellsElectron Microscope TomographyHIV-1HIV InfectionsMice, SCIDMicroscopyMicroscopy, FluorescenceViral Loadelectron tomographyHIV-1human biologyhumanized mouseinfectious diseaselight sheet microscopymedicinemicrobiologymousetissue clearing

Identifiers

PMID31657719
PMCPMC6839903
OpenAlexW2981999908

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.