ArticleJournal of visualized experiments : JoVE2019
Isolation and Differentiation of Primary Myoblasts from Mouse Skeletal Muscle Explants.
Article in Journal of visualized experiments : JoVE, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
8 citing papers in PubMed.
- Spatial proteomics identifies FXYD6 as a dual-site protein of neuromuscular junction in the diaphragm.Communications biology · 2026Article
- Aligned Collagen Sponges Loaded With Myogenic Factors to Enhance Skeletal Muscle Tissue Regeneration.Journal of biomedical materials research. Part B, Applied biomaterials · 2025Article
- Simplifying the protocol for low-pollution-risk, efficient mouse myoblast isolation and differentiation.Advanced biotechnology · 2025Article
- Functional dissection of metabolic trait-associated gene regulation in steady state and stimulated human skeletal muscle cells.bioRxiv : the preprint server for biology · 2025Article
- Derivation and long-term maintenance of porcine skeletal muscle progenitor cells.Scientific reports · 2024Article
- Laminin-221-derived recombinant fragment facilitates isolation of cultured skeletal myoblasts.Regenerative therapy · 2022Article
- KLB dysregulation mediates disrupted muscle development in intrauterine growth restriction.The Journal of physiology · 2022Article
- Neuromuscular disease modeling on a chip.Disease models & mechanisms · 2020Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
Abstract
Primary myoblasts are undifferentiated proliferating precursors of skeletal muscle. They can be cultured and studied as muscle precursors or induced to differentiate into later stages of muscle development. The protocol provided here describes a robust method for the isolation and culture of a highly proliferative population of myoblast cells from young adult mouse skeletal muscle explants. These cells are useful for the study of the metabolic properties of skeletal muscle of different mouse models, as well as in other downstream applications such as transfection with exogenous DNA or transduction with viral expression vectors. The level of differentiation and metabolic profile of these cells depends on the length of exposure, and composition of the media used to induce myoblast differentiation. These methods provide a robust system for the study of mouse muscle cell metabolism ex vivo. Importantly, unlike in vivo models, the methods described here provide a cell population that can be expanded and studied with high levels of reproducibility.
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What Socratic holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.