Evidence map›Paper›PMID 31687396›Full record

ArticleBioMed research international2019

Effects of Pyrene on Human Liver HepG2 Cells: Cytotoxicity, Oxidative Stress, and Transcriptomic Changes in Xenobiotic Metabolizing Enzymes and Inflammatory Markers with Protection Trial Using Lycopene.

Jin-Kui Ma, Walaa Fathy Saad Eldin, Waleed Rizk El-Ghareeb, Abdelazim Elsayed Elhelaly, Mariam H E Khedr, Xiang Li, Xiao-Chen Huang

Open access · hybridAbstract read
In one paragraph

Article in BioMed research international, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
1.3field-weighted citation impact, top 23% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 29 citations in OpenAlex.

  1. Article
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  4. Pharmaceutics · 2022
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 4 institutions in 4 countries.

Jin-Kui MaSchool of Food & Pharmaceutical Engineering, Zhaoqing University, Zhaoqing 526061, China.ORCID https://orcid.org/0000-0001-9346-2076
Walaa Fathy Saad EldinEducational Veterinary Hospital, Faculty of Veterinary Medicine, Zagazig University, Zagazig 44519, Egypt.ORCID https://orcid.org/0000-0002-1310-2212
Waleed Rizk El-GhareebDepartment of Veterinary Public Health and Animal Husbandry, College of Veterinary Medicine, King Faisal University, Al Hofuf, Saudi Arabia.ORCID https://orcid.org/0000-0001-6006-408X
Abdelazim Elsayed ElhelalyDepartment of Food Hygiene and Control, Faculty of Veterinary Medicine, Suez Canal University, Ismailia 41522, Egypt.
Mariam H E KhedrDepartment of Veterinary Hygiene, Faculty of Veterinary Medicine, Zagazig University, Zagazig 44519, Egypt.
Xiang LiCollege of Environmental and Chemical Engineering, Zhaoqing University, Zhaoqing 526061, China.
Xiao-Chen HuangSchool of Food & Pharmaceutical Engineering, Zhaoqing University, Zhaoqing 526061, China.ORCID https://orcid.org/0000-0002-5473-7222
Zhaoqing University · CNZagazig University · EGKing Faisal University · SASuez Canal University · EG

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Pyrene is one of the major polycyclic aromatic hydrocarbons formed during heat treatment of meat and in car exhausts; however, few studies have investigated pyrene-induced adverse effects on human cell lines. This study aimed at the investigation of pyrene-induced cytotoxicity and oxidative damage in human liver HepG2 cells at environmentally relevant concentrations. Pyrene-induced changes in mRNA expression of xenobiotic metabolizing enzymes (XMEs), xenobiotic transporters, antioxidant enzymes, and inflammatory markers were investigated using real-time PCR. As a protection trial, the ameliorative effects of lycopene, a carotenoid abundantly found in tomato, were investigated. The possible mechanisms behind such effects were examined via studying the co exposure effects of pyrene and lycopene on regulatory elements including the aryl hydrocarbon receptor (Air) and elytroid 2-related factor 2 (RF). The achieved results indicated that pyrene caused significant cytotoxicity at 50 n, with a clear production of reactive oxygen species (ROS) in a dose-dependent manner. Pyrene upregulated mRNA expression of phase I enzymes including CYP1A1, 1A2, and CYP1B1 and inflammatory markers including TNF

Indexed as

AntioxidantsBiomarkers, TumorCarotenoidsCytotoxinsDown-RegulationHep G2 CellsHumansInactivation, MetabolicInflammationLiverLycopeneOxidation-ReductionOxidative StressPolycyclic Aromatic HydrocarbonsPyrenesReactive Oxygen SpeciesAntioxidantsBiomarkers, TumorCarotenoidsCytotoxinsLycopenePolycyclic Aromatic HydrocarbonspyrenePyrenesReactive Oxygen SpeciesReceptors, Aryl HydrocarbonRNA, MessengerXenobiotics

Identifiers

PMID31687396
PMCPMC6803749
OpenAlexW2979588187

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.