Evidence map›Paper›PMID 31710099›Full record

ArticleEuropean journal of immunology2020

Graded diacylglycerol kinases α and ζ activities ensure mucosal-associated invariant T-cell development in mice.

Yun Pan, Wenhai Deng, Jinhai Xie, Shimeng Zhang, Edwin C K Wan, Lei Li, Huishan Tao, Zhiming Hu, Yongping Chen, Li Ma and 2 more

Open access · greenAbstract read
In one paragraph

Article in European journal of immunology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
0.4field-weighted citation impact, top 37% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 9 citations in OpenAlex.

  1. Article
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  6. DGK α and ζ Activities Control TFrontiers in immunology · 2019
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 5 institutions in 2 countries.

Yun PanDepartment of Pediatrics-Allergy and Immunology, Duke University Medical Center, Durham, NC.
Wenhai DengDepartment of Pediatrics-Allergy and Immunology, Duke University Medical Center, Durham, NC.
Jinhai XieSchool of Laboratory Medicine, Wenzhou Medical University, Wenzhou, Zhejiang Province, China.
Shimeng ZhangDepartment of Pediatrics-Allergy and Immunology, Duke University Medical Center, Durham, NC.
Edwin C K WanDepartment of Pediatrics-Allergy and Immunology, Duke University Medical Center, Durham, NC.
Lei LiDepartment of Pediatrics-Allergy and Immunology, Duke University Medical Center, Durham, NC.
Huishan TaoDepartment of Pediatrics-Allergy and Immunology, Duke University Medical Center, Durham, NC.
Zhiming HuDepartment of Pediatrics-Allergy and Immunology, Duke University Medical Center, Durham, NC.
Yongping ChenDepartment of Infectious Diseases, the First Affiliated Hospital of Wenzhou Medical University and Wenzhou Key Laboratory of Hepatology, Hepatology Institute of Wenzhou Medical University, Wenzhou, China.
Li MaInstitute of Molecular Immunology, School of Laboratory Medicine and Biotechnology, Southern Medical University, Guangzhou, China.
Jimin GaoSchool of Laboratory Medicine, Wenzhou Medical University, Wenzhou, Zhejiang Province, China.
Xiao-Ping ZhongDepartment of Pediatrics-Allergy and Immunology, Duke University Medical Center, Durham, NC.ORCID 0000-0002-4619-8783
Duke University · USWenzhou Medical University · CNUnion Hospital · CNSouthern Medical University · CNWest Virginia University · US

Funding

Regulation of peripheral T cell toleranceR01AI079088 · NIAID · DUKE UNIVERSITY · PI ZHONG, XIAOPING · 2008 to 2019
$3.5M
TSC1-mTOR signaling and T cell toleranceR01AI101206 · NIAID · DUKE UNIVERSITY · PI ZHONG, XIAOPING · 2012 to 2016
$1.9M
NIAID NIH HHS R01 AI079088NIAID NIH HHS R01 AI101206
6 · The paper itself

Abstract

Mucosal-associated invariant T (MAIT) cells participate in both protective immunity and pathogenesis of diseases. Most murine MAIT cells express an invariant TCRVα19-Jα33 (iVα19) TCR, which triggers signals crucial for their development. However, signal pathways downstream of the iVα19TCR and their regulation in MAIT cells are unknown. Diacylglycerol (DAG) is a critical second messenger that relays the TCR signal to multiple downstream signaling cascades. DAG is terminated by DAG kinase (DGK)-mediated phosphorylation and conversion to phosphatidic acid. We have demonstrated here that downregulation of DAG caused by enhanced DGK activity impairs late-stage MAIT cell maturation in both thymus and spleen. Moreover, deficiency of DGKζ but not DGKα by itself causes modest decreases in MAIT cells, and deficiency of both DGKα and ζ results in severe reductions of MAIT cells in an autonomous manner. Our studies have revealed that DAG signaling is not only critical but also must be tightly regulated by DGKs for MAIT cell development and that both DGKα and, more prominently, DGKζ contribute to the overall DGK activity for MAIT cell development.

Indexed as

AnimalsDiacylglycerol KinaseDiglyceridesMiceMucosal-Associated Invariant T CellsSignal Transduction1,2-diacylglycerolDiacylglycerol KinaseDiglyceridesDGKαDGKζDiacylglycerol kinasesMAIT cellsMucosal-associated invariant T cellsSignal transduction

Identifiers

PMID31710099
PMCPMC7249235
OpenAlexW2983206998

What Socratic holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.