ArticleThe Analyst2020
Fourier transform infrared spectroscopy of developing bone mineral: from amorphous precursor to mature crystal.
Article in The Analyst, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed, 50 citations in OpenAlex.
- Preparation of amorphous calcium phosphate nanoparticles using small organic molecules for biomimetic mineralization of dentin.RSC advances · 2026Article
- Engineering macromolecular crowding-driven amyloid-mineral hybrid scaffolds for enhanced bone regeneration.Bioactive materials · 2026Article
- Modulation of TNAP activity and apatite formation in biomimetic matrix vesicles studied byBiochimica et biophysica acta. Biomembranes · 2025Article
- Synchrotron FTIR microspectroscopy for quantitative assessment of collagen mineralization: a comparative analytical study.Analytical and bioanalytical chemistry · 2025Article
- Semi-quantitative evaluation of small organic molecules content in amorphous calcium phosphate.Scientific reports · 2025Article
- Mesoscale orchestration of collagen-based hierarchical mineralization.Nature communications · 2025Article
- Article
- Physical-Mechanical Properties and Mineral Deposition of a Pit-and-Fissure Sealant Containing Niobium-Fluoride Nanoparticles-An In Vitro Study.Materials (Basel, Switzerland) · 2024Article
- The Ways of Forming and the Erosion/Decay/Aging of Bioapatites in the Context of the Reversibility of Apatites.International journal of molecular sciences · 2024Article
- Incorporating strontium enriched amorphous calcium phosphate granules in collagen/collagen-magnesium-hydroxyapatite osteochondral scaffolds improves subchondral bone repair.Materials today. Bio · 2024Article
- Low-Temperature Plasmas Improving Chemical and Cellular Properties of Poly (Ether Ether Ketone) Biomaterial for Biomineralization.Materials (Basel, Switzerland) · 2023Article
- Article
- A Bioinspired Gelatin-Amorphous Calcium Phosphate Coating on Titanium Implant for Bone Regeneration.Advanced healthcare materials · 2023Article
- FTIR spectrum analysis to predict the crystalline and amorphous phases of hydroxyapatite: a comparison of vibrational motion to reflection.RSC advances · 2023Article
- Small organic molecules containing amorphous calcium phosphate: synthesis, characterization and transformation.Frontiers in bioengineering and biotechnology · 2023Article
- Article
- Surface and Structural Studies of Age-Related Changes in Dental Enamel: An Animal Model.Materials (Basel, Switzerland) · 2022Article
- A previously unknown way of heme detoxification in the digestive tract of cats.Scientific reports · 2021Article
- Applications of Vibrational Spectroscopy for Analysis of Connective Tissues.Molecules (Basel, Switzerland) · 2021Review
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
Abstract
Bone mineral development has been described to proceed through an amorphous precursor prior to apatite crystallization. However, further analytical approaches are necessary to identify specific markers of amorphous mineral components in bone. Here, we establish an original Fourier transform infrared (FTIR) spectroscopy approach to allow the specific identification of the amorphous and/or crystalline nature of bone mineral. Using a series of standards, our results demonstrate that obtaining the second derivative of the FTIR spectra could reveal a peak specifically corresponding to amorphous calcium phosphate (ACP) at ∼992 cm-1. The intensity of this peak was strongly correlated to ACP content in standard mixtures. The analysis of a variety of bones showed that a clear ACP peak could be identified as a specific marker of the existence of an amorphous mineral component in developing bones. In contrast, the ACP peak was not detected in the mature bones. Moreover, subjecting developing bones to ex vivo crystallization conditions led to a clear reduction of the ACP peak, further substantiating the conversion of amorphous mineral precursor into mature apatite crystals. Analysis of mineralization in osteogenic cell cultures corroborated our observations, showing the presence of ACP as a major transient component in early mineralization, but not in the mature matrix. Additionally, FTIR imaging revealed that ACP was present in areas of matrix development, distributed around the edges of mineralizing nodules. Using an original analytical approach, this work provides strong evidence to support that bone mineral development is initiated by an amorphous precursor prior to apatite crystallization.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.