Evidence map›Paper›PMID 31762963›Full record

ArticleJournal of extracellular vesicles2019

Biological membranes in EV biogenesis, stability, uptake, and cargo transfer: an ISEV position paper arising from the ISEV membranes and EVs workshop.

Ashley E Russell, Alexandra Sneider, Kenneth W Witwer, Paolo Bergese, Suvendra N Bhattacharyya, Alexander Cocks, Emanuele Cocucci, Uta Erdbrügger, Juan M Falcon-Perez, David W Freeman and 27 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of extracellular vesicles, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 164 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
164citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

164 citing papers in PubMed, 1 synthesis or guideline pooled it.

  1. Pooled it
  2. Review
  3. Review
  4. The relevance of membrane lipid composition in exosome-based precision dermatology.Journal of the European Academy of Dermatology and Venereology : JEADV · 2026
    Article
  5. Article
  6. Review
  7. Article
  8. Review
  9. Article
  10. Article
  11. Article
  12. Review
  13. Updating MISEV: When, What, How, and Why?Journal of extracellular vesicles · 2026
    Article
  14. Article
  15. Article
  16. Review
  17. Review
  18. Adipose-Derived Extracellular Vesicles and Intercellular Crosstalk With Skeletal Muscle: Implications for Sarcopenic Obesity and Metabolic Dysregulation.Obesity reviews : an official journal of the International Association for the Study of Obesity · 2026
    Review
  19. Article
  20. Article

104 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

37 authors.

Ashley E RussellDepartment of Molecular and Comparative Pathobiology, The Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Alexandra SneiderDepartment of Chemical and Biomolecular Engineering, Johns Hopkins University, Baltimore, MD, USA.
Kenneth W WitwerDepartment of Molecular and Comparative Pathobiology, The Johns Hopkins University School of Medicine, Baltimore, MD, USA.ORCID 0000-0003-1664-4233
Paolo BergeseDepartment of Molecular and Translational Medicine, Università degli Studi di Brescia, CSGI and INSTM, Brescia, Italy.ORCID 0000-0002-4652-2168
Suvendra N BhattacharyyaMolecular Genetics Division, CSIR-Indian Institute of Chemical Biology, Kolkata, India.
Alexander CocksCardiff University, School of Medicine, Cardiff, UK.
Emanuele CocucciDivision of Pharmaceutics and Pharmacology, College of Pharmacy, Columbus, OH, USA.ORCID 0000-0002-0088-9845
Uta ErdbrüggerUniversity of Virginia, Charlottesville, VA, USA.
Juan M Falcon-PerezExosomes laboratory and Metabolomics Platform, CIC bioGUNE, CIBERehd, Bizkaia, Spain.
David W FreemanLaboratory of Epidemiology and Population Science, National Institute on Aging, National Institutes of Health, Baltimore, MD, USA.
Thomas M GallagherDepartment of Microbiology and Immunology, Loyola University Chicago, Chicago, IL, USA.
Shuaishuai HuSchool of Biological and Healthy Sciences, Technological University Dublin, Dublin, Ireland.
Yiyao HuangDepartment of Molecular and Comparative Pathobiology, The Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Steven M JayFischell Department of Bioengineering, University of Maryland, College Park, MD, USA.ORCID 0000-0002-3827-5988
Shin-Ichi KanoDepartment of Psychiatry and Behavioral Neurobiology, The University of Alabama at Birmingham School of Medicine, Birmingham, AL, USA.
Gregory LavieuINSERM U932, Institut Curie, PSL Research University, France.
Aleksandra LeszczynskaDepartment of Pediatrics, University of California San Diego, La Jolla, CA, USA.
Alicia M LlorenteDepartment of Molecular Cell Biology, Institute for Cancer Research, Oslo University Hospital, Oslo, Norway.
Quan LuProgram in Molecular and Integrative Physiological Sciences Departments of Environmental Health, Genetics & Complex Diseases Harvard T.H. Chan School of Public Health, Boston, MA, USA.
Vasiliki MahairakiDepartment of Neurology, Johns Hopkins School of Medicine, Baltimore, MD, USA.
Dillon C MuthDepartment of Molecular and Comparative Pathobiology, The Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Nicole Noren HootenLaboratory of Epidemiology and Population Science, National Institute on Aging, National Institutes of Health, Baltimore, MD, USA.ORCID 0000-0002-1683-3838
Matias OstrowskiINBIRS Institute, UBA-CONICET School of Medicine University of Buenos Aires, Buenos Aires, Argentina.
Ilaria PradaCNR Institute of Neuroscience, Milan, Italy.
Susmita SahooCardiovascular Research Center, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
Tine Hiorth SchøyenDepartment of Molecular and Comparative Pathobiology, The Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Lifu ShengDepartment of Pathology, University of Washington School of Medicine, Seattle, WA, USA.
Deanna TeschDepartment of Chemistry, Shaw University, Raleigh, NC, USA.
Guillaume Van NielInstitute for Psychiatry and Neuroscience of Paris, INSERM U1266, Hopital Saint-Anne, Université Descartes, Paris, France.
Roosmarijn E VandenbrouckeVIB Center for Inflammation Research, Ghent, Belgium.ORCID 0000-0002-8327-620X
Frederik J VerweijInstitute for Psychiatry and Neuroscience of Paris, INSERM U1266, Hopital Saint-Anne, Université Descartes, Paris, France.
Ana V VillarInstituto de Biomedicina y Biotecnología de Cantabria (IBBTEC) CSIC-Universidad de Cantabria and Departamento de Fisiología y Farmacología, Universidad de Cantabria, Santander, Spain.
Marca WaubenDepartment of Biochemistry and Cell Biology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands.
Ann M WehmanRudolf Virchow Center, Julius-Maximilians-Universität Würzburg, Würzburg, Germany.ORCID 0000-0001-9826-4132
Hang YinSchool of Pharmaceutical Sciences, Tsinghua University-Peking University Joint Center for Life Sciences, Tsinghua University, Beijing, China.
David Raul Francisco CarterDepartment of Biological and Medical Sciences, Oxford Brookes University, Oxford, UK.
Pieter VaderLaboratory of Clinical Chemistry and Haematology & Department of Experimental Cardiology, University Medical Center Utrecht, Utrecht, The Netherlands.ORCID 0000-0002-7059-8920

Funding

Translational Research Support CoreP30ES000002 · NIEHS · HARVARD UNIVERSITY (SCH OF PUBLIC HLTH) · PI JAIME ELIZABETH HART · 1985 to 2026
$44.6M
Training Veterinarians for Careers in Biomedical ResearchT32OD011089 · OD · JOHNS HOPKINS UNIVERSITY · PI JOSEPH L MANKOWSKI · 2012 to 2026
$6.7M
Extracellular Vesicle-Encapsulated AAVs for Therapeutic Gene Delivery to the HeartR01HL148786 · NHLBI · ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI · PI Kiyotake Ishikawa, Susmita Sahoo · 2019 to 2026
$4.1M
Next-generation extracellular vesicle biologics to target central nervous system and peripheral reservoirs of HIVR01AI144997 · NIAID · JOHNS HOPKINS UNIVERSITY · PI RAMRATNAM, BHARAT, WITWER, KENNETH W · 2019 to 2023
$4.0M
Extracellular Vesicle and Extracellular RNA Biomarkers of HIV-1 Central Nervous System Pathogenesis and Cigarette UseR01DA047807 · NIDA · JOHNS HOPKINS UNIVERSITY · PI WITWER, KENNETH W · 2018 to 2022
$3.7M
Stroke and Alzheimers Disease Related DementiasT32AG052375 · NIA · WEST VIRGINIA UNIVERSITY · PI Paul D Chantler, BERNARD G. SCHREURS · 2017 to 2026
$3.5M
Prenatal metal mixtures and neurodevelopment: Role of placental extracellular microRNAsR01ES030302 · NIEHS · ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI · PI LU, QUAN, WRIGHT, ROSALIND J · 2019 to 2023
$3.2M
Predictive and systems modeling of exosome cargoR01HL145644 · NHLBI · EMORY UNIVERSITY · PI DAVIS, MICHAEL E · 2019 to 2022
$3.1M
SPP1, Oxidative Stress, and Lead ToxicityR01ES029097 · NIEHS · HARVARD SCHOOL OF PUBLIC HEALTH · PI LU, QUAN · 2018 to 2022
$2.8M
Extracellular Vesicle-Mediated Immunomodulation in HIV Infection and Nicotine AbuseR01DA040385 · NIDA · JOHNS HOPKINS UNIVERSITY · PI WITWER, KENNETH W · 2015 to 2019
$2.3M
Impact of immune cell-derived exosomes and miRNAs on brain function and behaviorR01MH113645 · NIMH · UNIVERSITY OF ALABAMA AT BIRMINGHAM · PI KANO, SHINICHI · 2018 to 2022
$2.2M
MicroRNA-10a in Airway Smooth Muscle and AsthmaR01HL139496 · NHLBI · HARVARD SCHOOL OF PUBLIC HEALTH · PI LU, QUAN · 2018 to 2021
$2.1M
NCI NIH HHS UG3 CA241694NHLBI NIH HHS K23 HL126101NHLBI NIH HHS R01 HL124187NHLBI NIH HHS R01 HL139496NHLBI NIH HHS R01 HL140469NHLBI NIH HHS R01 HL141611NHLBI NIH HHS R01 HL145644NHLBI NIH HHS R01 HL148786NIAID NIH HHS R01 AI144997NIA NIH HHS R56 AG057430NIA NIH HHS T32 AG052375NIDA NIH HHS R01 DA040385NIDA NIH HHS R01 DA047807NIEHS NIH HHS P30 ES000002NIEHS NIH HHS R01 ES029097NIEHS NIH HHS R01 ES030302NIGMS NIH HHS R01 GM130923NIH HHS T32 OD011089NIMH NIH HHS R01 MH113645NIMH NIH HHS R21 MH118164
6 · The paper itself

Abstract

Paracrine and endocrine roles have increasingly been ascribed to extracellular vesicles (EVs) generated by multicellular organisms. Central to the biogenesis, content, and function of EVs are their delimiting lipid bilayer membranes. To evaluate research progress on membranes and EVs, the International Society for Extracellular Vesicles (ISEV) conducted a workshop in March 2018 in Baltimore, Maryland, USA, bringing together key opinion leaders and hands-on researchers who were selected on the basis of submitted applications. The workshop was accompanied by two scientific surveys and covered four broad topics: EV biogenesis and release; EV uptake and fusion; technologies and strategies used to study EV membranes; and EV transfer and functional assays. In this ISEV position paper, we synthesize the results of the workshop and the related surveys to outline important outstanding questions about EV membranes and describe areas of consensus. The workshop discussions and survey responses reveal that while much progress has been made in the field, there are still several concepts that divide opinion. Good consensus exists in some areas, including particular aspects of EV biogenesis, uptake and downstream signalling. Areas with little to no consensus include EV storage and stability, as well as whether and how EVs fuse with target cells. Further research is needed in these key areas, as a better understanding of membrane biology will contribute substantially towards advancing the field of extracellular vesicles.

Indexed as

biogenesisExosomesExtracellular vesiclesfusionISEV workshopmembranesposition papertechnologyuptake

Identifiers

PMID31762963
PMCPMC6853251

What Socratic holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.