ArticleGenome research2019
Systematic genome editing of the genes on zebrafish Chromosome 1 by CRISPR/Cas9.
Article in Genome research, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
What it found
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Who cites it
32 citing papers in PubMed, 49 citations in OpenAlex.
- A decoy receptor antagonizes interferon mediated antiviral responses in teleost fish.BMC biology · 2026Article
- Efficient recovery of zebrafish maternal-zygotic mutants from in vivo expanded and cryopreserved germline stem cells.Communications biology · 2026Article
- Stn1 promotes zebrafish oocyte development via amplifying Wnt/β-catenin signaling.EMBO reports · 2026Article
- Tead1a Initiates Transcriptional Priming Through the TEAD1a/YAP-Notch1-Spi1/Cebpα Axis to Promote Neutrophil Fate.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- doc2a and doc2b contribute to locomotor and social behaviors by down-regulating npas4b in zebrafish.BMC biology · 2025Article
- Article
- Ddx3xa mutations drive cardiac defects in a zebrafish model via dysregulation of wnt/β-catenin signaling.Frontiers in molecular biosciences · 2025Article
- Stochastic Epigenetic Modification and Evolution of Sex Determination in Vertebrates.Journal of molecular evolution · 2024Article
- High-throughput and genome-scale targeted mutagenesis using CRISPR in a nonmodel multicellular organism,Genome research · 2024Article
- Scalable CRISPR Screens in Zebrafish Using MIC-Drop.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Induced formation of primordial germ cells from zebrafish blastomeres by germplasm factors.Nature communications · 2023Article
- Article
- Upf3a but not Upf1 mediates the genetic compensation response induced by leg1 deleterious mutations in an H3K4me3-independent manner.Cell discovery · 2023Article
- Circadian regulation of developmental synaptogenesis via the hypocretinergic system.Nature communications · 2023Article
- Large-scale F0 CRISPR screens in vivo using MIC-Drop.Nature protocols · 2023Review
- Review
- Depletion ofZoological research · 2023Article
- Atf7ip and Setdb1 interaction orchestrates the hematopoietic stem and progenitor cell state with diverse lineage differentiation.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Article
- Identification of fish spermatogenic cells through high-throughput immunofluorescence against testis with an antibody set.Frontiers in endocrinology · 2023Article
Corrections and comments
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Authors and funding
37 authors at 18 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Genome editing by the well-established CRISPR/Cas9 technology has greatly facilitated our understanding of many biological processes. However, a complete whole-genome knockout for any species or model organism has rarely been achieved. Here, we performed a systematic knockout of all the genes (1333) on Chromosome 1 in zebrafish, successfully mutated 1029 genes, and generated 1039 germline-transmissible alleles corresponding to 636 genes. Meanwhile, by high-throughput bioinformatics analysis, we found that sequence features play pivotal roles in effective gRNA targeting at specific genes of interest, while the success rate of gene targeting positively correlates with GC content of the target sites. Moreover, we found that nearly one-fourth of all mutants are related to human diseases, and several representative CRISPR/Cas9-generated mutants are described here. Furthermore, we tried to identify the underlying mechanisms leading to distinct phenotypes between genetic mutants and antisense morpholino-mediated knockdown embryos. Altogether, this work has generated the first chromosome-wide collection of zebrafish genetic mutants by the CRISPR/Cas9 technology, which will serve as a valuable resource for the community, and our bioinformatics analysis also provides some useful guidance to design gene-specific gRNAs for successful gene editing.
Identifiers
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.