Evidence mapPaperPMID 31856839Full record

ArticleLipids in health and disease2019

Sequence analysis and variant identification at the APOC3 gene locus indicates association of rs5218 with BMI in a sample of Kuwaiti's.

Zainab H Malalla, Ahmad E Al-Serri, Huda M AlAskar, Wafaa Y Al-Kandari, Suzanne A Al-Bustan

Open access · goldAbstract read
In one paragraph

Article in Lipids in health and disease, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.5field-weighted citation impact, top 26% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 7 citations in OpenAlex.

  1. Article
  2. Review
  3. Sequence Variant Analysis of theInternational journal of molecular sciences · 2023
    Article
4 · The record

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5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Zainab H MalallaDepartment of Biological Sciences, Faculty of Science, Kuwait University, Kuwait, Kuwait.
Ahmad E Al-SerriHuman Genetics Unit, Department of Pathology, Faculty of Medicine, Kuwait University, Hawally, Kuwait.
Huda M AlAskarDepartment of Biological Sciences, Faculty of Science, Kuwait University, Kuwait, Kuwait.
Wafaa Y Al-KandariDepartment of Biological Sciences, Faculty of Science, Kuwait University, Kuwait, Kuwait.
Suzanne A Al-BustanDepartment of Biological Sciences, Faculty of Science, Kuwait University, Kuwait, Kuwait. s.albustan@ku.edu.kw.ORCID https://orcid.org/0000-0002-1260-4632
Kuwait University · KW

Funding

College of Graduate Studies, Kuwait University n/aKuwait University YS01/12
6 · The paper itself

Abstract

backgroundAPOC3 is important in lipid transport and metabolism with limited studies reporting genetic sequence variations in specific ethnic groups. The present study aimed to analyze the full APOC3 sequence among Kuwaiti Arabs and test the association of selected variants with lipid levels and BMI.

methodsVariants were identified by Sanger sequencing the entire APOC3 gene in 100 Kuwaiti Arabs. Variants and their genotypes were fully characterized and used to construct haplotype blocks. Four variants (rs5128, rs2854117, rs2070668, KUAPOC3N3 g.5196 A > G) were selected for testing association with serum lipid levels and BMI in a cohort (n = 733).

resultsAPOC3 sequence (4.3 kb) of a Kuwaiti Arab was deposited in Genbank (accession number KJ437193). Forty-two variants including 3 novels were identified including an "A" insertion at genomic positions 116,700,599-116,700,600 (promoter region) and two substitutions in intron 1 at genomic positions 116,700,819 and 116,701,159. Only three variants, (rs5128, rs2854117, and rs2070668) were analyzed for association of which rs5128 showed a trend for association with increased BMI, TG and VLDL levels that was further investigated using multivariate analysis. A significant association of rs5128 with BMI (p <  0.05) was observed following a dominant genetic model with increased risk by an OR of 4.022 (CI: 1.13-14.30).

conclusionThe present study is the first to report sequence analysis of APOC3 in an Arab ethnic group. This study supports the inclusion of rs5128 as a marker for assessing genetic risk to dyslipidemia and obesity and the inclusion of the novel variant g.5196 A > G for population stratification of Arabs.

Indexed as

Body Mass IndexGenetic Association StudiesGenetic Predisposition to DiseaseAdolescentAdultAgedApolipoprotein C-IIIArabsFemaleGenotypeHumansLipid MetabolismLipoproteins, VLDLMaleMiddle AgedPolymorphism, Single NucleotideAPOC3 protein, humanApolipoprotein C-IIILipoproteins, VLDLTriglyceridesAPOC3ArabsBMIGenetic associationLipid levelsSequence variants

Identifiers

PMID31856839
PMCPMC6921598
OpenAlexW2995822879

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.