In Situ Proteolysis Condition-Induced Crystallization of the XcpVWX Complex in Different Lattices.

Yichen Zhang, Shu Wang, Zongchao Jia

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In one paragraph

Article in International journal of molecular sciences, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

0.1field-weighted citation impact, top 68% of its field

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 2 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 2 institutions in 2 countries.

Yichen ZhangDepartment of Biomedical and Molecular Sciences, Queen's University, 18 Stuart Street, Kingston, ON K7L 3N6, Canada.

Shu WangCollege of Chemistry, Beijing Normal University, 19 Xinjiekou Outer Street, Beijing 100875, China.

Zongchao JiaDepartment of Biomedical and Molecular Sciences, Queen's University, 18 Stuart Street, Kingston, ON K7L 3N6, Canada.ORCID 0000-0002-9322-9394

Queen's University · CABeijing Normal University · CN

Funding

Natural Sciences and Engineering Research Council of Canada RGPIN-2018-04427

6 · The paper itself

Abstract

Although prevalent in the determination of protein structures; crystallography always has the bottleneck of obtaining high-quality protein crystals for characterizing a wide range of proteins; especially large protein complexes. Stable fragments or domains of proteins are more readily to crystallize; which prompts the use of in situ proteolysis to remove flexible or unstable structures for improving crystallization and crystal quality. In this work; we investigated the effects of in situ proteolysis by chymotrypsin on the crystallization of the XcpVWX complex from the Type II secretion system of

Indexed as

ChymotrypsinBacterial ProteinsCrystallizationCrystallography, X-RayMembrane ProteinsMultiprotein ComplexesProtein ConformationProteolysisType II Secretion SystemsBacterial ProteinsChymotrypsinMembrane ProteinsMultiprotein ComplexesType II Secretion SystemsXcpV protein, Pseudomonas aeruginosaXcpW protein, Pseudomonas aeruginosaXcpX protein, Pseudomonas aeruginosachymotrypsin digestionin situ proteolysispseudopilin tip complextype II secretion systemX-ray crystallography

Identifiers

PMID31906428

DOI10.3390/ijms21010308

PMCPMC6981927

OpenAlexW2997345104

What Socratic holds

Textmetadata

LicenceCC BY

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.