Evidence map›Paper›PMID 31941923›Full record

ArticleScientific reports2020

Exosomes released upon mitochondrial ASncmtRNA knockdown reduce tumorigenic properties of malignant breast cancer cells.

Lorena Lobos-González, Rocío Bustos, América Campos, Valeria Silva, Verónica Silva, Emanuel Jeldes, Carlos Salomon, Manuel Varas-Godoy, Albano Cáceres-Verschae, Eduardo Duran and 5 more

Open access · goldAbstract read
In one paragraph

Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.

0numbers the graph read from it
0cells of the map it votes in
18citing papers in PubMed
1.3field-weighted citation impact, top 22% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

18 citing papers in PubMed, 29 citations in OpenAlex.

  1. Review
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  9. Human mtDNA-Encoded Long ncRNAs: Knotty Molecules and Complex Functions.International journal of molecular sciences · 2024
    Review
  10. Article
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  17. The Advancing Roles of Exosomes in Breast Cancer.Frontiers in cell and developmental biology · 2021
    Review
  18. Mitochondria Encoded Non-coding RNAs in Cell Physiology.Frontiers in cell and developmental biology · 2021
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors at 7 institutions in 3 countries.

Lorena Lobos-GonzálezCentro de Medicina Regenerativa, Facultad de Medicina, Clínica Alemana-Universidad del Desarrollo, Santiago, Chile. lorotae@gmail.com.
Rocío BustosAndes Biotechnologies/Fundación Ciencia & Vida, Santiago, Chile.
América CamposCentro de Estudios Avanzados de Enfermedades Crónicas (ACCDIS), Santiago, Chile.
Valeria SilvaAndes Biotechnologies/Fundación Ciencia & Vida, Santiago, Chile.
Verónica SilvaCentro de Medicina Regenerativa, Facultad de Medicina, Clínica Alemana-Universidad del Desarrollo, Santiago, Chile.
Emanuel JeldesAndes Biotechnologies/Fundación Ciencia & Vida, Santiago, Chile.
Carlos SalomonExosome Biology Laboratory, Centre for Clinical Diagnostics, University of Queensland Centre for Clinical Research, Royal Brisbane and Women's Hospital, The University of Queensland, Brisbane, Australia.ORCID http://orcid.org/0000-0003-4474-0046
Manuel Varas-GodoyCancer Cell Biology Lab., Centro de Biología Celular y Biomedicina (CEBICEM), Facultad de Medicina y Ciencia, Universidad San Sebastián, Santiago, Chile.
Albano Cáceres-VerschaeDoctorado en Biomedicina, Centro de investigación e innovación Biomedica, Universidad de los Andes, Santiago, Chile.ORCID http://orcid.org/0000-0002-4057-6827
Eduardo DuranCentro de Medicina Regenerativa, Facultad de Medicina, Clínica Alemana-Universidad del Desarrollo, Santiago, Chile.
Tamara VeraCentro de Medicina Regenerativa, Facultad de Medicina, Clínica Alemana-Universidad del Desarrollo, Santiago, Chile.
Fernando EzquerCentro de Medicina Regenerativa, Facultad de Medicina, Clínica Alemana-Universidad del Desarrollo, Santiago, Chile.
Marcelo EzquerCentro de Medicina Regenerativa, Facultad de Medicina, Clínica Alemana-Universidad del Desarrollo, Santiago, Chile.
Verónica A BurzioAndes Biotechnologies/Fundación Ciencia & Vida, Santiago, Chile.ORCID http://orcid.org/0000-0002-6491-4640
Jaime VillegasAndes Biotechnologies/Fundación Ciencia & Vida, Santiago, Chile.
Universidad del Desarrollo · CLUniversidad Andrés Bello · CLSan Sebastián University · CLThe University of Queensland · AUUniversidad Andrés Bello · CLUniversidad de Los Andes, Chile · CLUniversity of Chile · CL

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

During intercellular communication, cells release extracellular vesicles such as exosomes, which contain proteins, ncRNAs and mRNAs that can influence proliferation and/or trigger apoptosis in recipient cells, and have been proposed to play an essential role in promoting invasion of tumor cells and in the preparation of metastatic niches. Our group proposed the antisense non-coding mitochondrial RNA (ASncmtRNA) as a new target for cancer therapy. ASncmtRNA knockdown using an antisense oligonucleotide (ASO-1537S) causes massive death of tumor cells but not normal cells and strongly reduces metastasis in mice. In this work, we report that exosomes derived from ASO-1537S-treated MDA-MB-231 breast cancer cells (Exo-1537S) inhibits tumorigenesis of recipient cells, in contrast to exosomes derived from control-ASO-treated cells (Exo-C) which, in contrast, enhance these properties. Furthermore, an in vivo murine peritoneal carcinomatosis model showed that Exo-1537S injection reduced tumorigenicity compared to controls. Proteomic analysis revealed the presence of Lactadherin and VE-Cadherin in exosomes derived from untreated cells (Exo-WT) and Exo-C but not in Exo-1537S, and the latter displayed enrichment of proteasomal subunits. These results suggest a role for these proteins in modulation of tumorigenic properties of exosome-recipient cells. Our results shed light on the mechanisms through which ASncmtRNA knockdown affects the preparation of breast cancer metastatic niches in a peritoneal carcinomatosis model.

Indexed as

AnimalsAntigens, CDAntigens, SurfaceBreast NeoplasmsCadherin 5CadherinsCarcinogenesisCell Line, TumorCell MovementCell ProliferationExosomesFemaleHumansMiceMilk ProteinsMitochondriaAntigens, CDAntigens, SurfaceCadherin 5CadherinsMFGE8 protein, humanMilk ProteinsOligoribonucleotides, AntisenseRNA, Untranslated

Identifiers

PMID31941923
PMCPMC6962334
OpenAlexW2999910062

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.