Evidence map›Paper›PMID 31951973›Full record

ArticleBiomaterials2020

Human lung organoids develop into adult airway-like structures directed by physico-chemical biomaterial properties.

Briana R Dye, Richard L Youngblood, Robert S Oakes, Tadas Kasputis, Daniel W Clough, Jason R Spence, Lonnie D Shea

Open access · greenAbstract read
In one paragraph

Article in Biomaterials, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers, 3 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
35citing papers in PubMed, 3 pooled it
5.6field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

35 citing papers in PubMed, 3 syntheses or guidelines pooled it, 69 citations in OpenAlex.

  1. Pooled it
  2. Pooled it
  3. Pooled it
  4. Review
  5. Review
  6. Review
  7. Review
  8. Multiscale Construction, Evaluation, and Application of Organoids.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025
    Review
  9. Article
  10. Review
  11. Review
  12. Review
  13. Engineering CAR-T Therapeutics for Enhanced Solid Tumor Targeting.Advanced materials (Deerfield Beach, Fla.) · 2025
    Review
  14. Review
  15. Article
  16. Exploiting Matrix Stiffness to Overcome Drug Resistance.ACS biomaterials science & engineering · 2024
    Review
  17. Article
  18. Review
  19. Engineered organoids for biomedical applications.Advanced drug delivery reviews · 2023
    Review
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Briana R DyeDepartment of Biomedical Engineering, USA.
Richard L YoungbloodDepartment of Biomedical Engineering, USA.
Robert S OakesDepartment of Biomedical Engineering, USA.
Tadas KasputisDepartment of Biomedical Engineering, USA.
Daniel W CloughDepartment of Biomedical Engineering, USA.
Jason R SpenceDepartment of Internal Medicine, University of Michigan, Ann Arbor, MI, USA.
Lonnie D SheaDepartment of Biomedical Engineering, USA. Electronic address: ldshea@umich.edu.
University of Michigan–Ann Arbor · US

Funding

Tissue Engineering and RegenerationT32DE007057 · NIDCR · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI DAVID H. KOHN · 1985 to 2026
$17.3M
TRAINING PROGRAM IN ORGANOGENESIST32HD007505 · NICHD · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI PASCA DI MAGLIANO, MARINA, SPENCE, JASON · 1997 to 2021
$6.9M
Supplement to a Hox-Wnt-Sox signaling axis regulates lung development, patterning and branchingR01HL119215 · NHLBI · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI SPENCE, JASON · 2013 to 2021
$4.2M
Protein-Releasing Microporous Scaffolds for Cell Replacement TherapyR01EB009910 · NIBIB · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI LUO, XUNRONG, SHEA, LONNIE D · 2010 to 2018
$3.5M
Cellular Biotechnology Training Program (CBTP) - Years 31-35T32GM145304 · NIGMS · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI Guizhi Zhu · 2022 to 2026
$2.6M
Establishing immunoprivileged scaffolds for transplantation of immature beta cellsR21AI147677 · NIAID · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI SHEA, LONNIE D, SHIRWAN, HAVAL · 2019 to 2020
$440k
Microporous scaffolds for enhancing efficiency of beta-cell progenitor maturation in vitro and in vivoR21EB024410 · NIBIB · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI SHEA, LONNIE D, SPENCE, JASON · 2017 to 2018
$400k
NHLBI NIH HHS R01 HL119215NIAID NIH HHS R21 AI147677NIBIB NIH HHS R01 EB009910NIBIB NIH HHS R21 EB024410NICHD NIH HHS T32 HD007505NIDCR NIH HHS T32 DE007057NIGMS NIH HHS T32 GM145304
6 · The paper itself

Abstract

Tissues derived from human pluripotent stem cells (hPSCs) often represent early stages of fetal development, but mature at the molecular and structural level when transplanted into immunocompromised mice. hPSC-derived lung organoids (HLOs) transplantation has been further enhanced with biomaterial scaffolds, where HLOs had improved tissue structure and cellular differentiation. Here, our goal was to define the physico-chemical biomaterial properties that maximally enhanced transplant efficiency, including features such as the polymer type, degradation, and pore interconnectivity of the scaffolds. We found that transplantation of HLOs on microporous scaffolds formed from poly (ethylene glycol) (PEG) hydrogel scaffolds inhibit growth and maturation, and the transplanted HLOs possessed mostly immature lung progenitors. On the other hand, HLOs transplanted on poly (lactide-co-glycolide) (PLG) scaffolds or polycaprolactone (PCL) led to tube-like structures that resembled both the structure and cellular diversity of an adult airway. Our data suggests that scaffold pore interconnectivity and polymer degradation contributed to the maturation, and we found that the size of the airway structures and the total size of the transplanted tissue was influenced by the material degradation rate. Collectively, these biomaterial platforms provide a set of tools to promote maturation of the tissues and to control the size and structure of the organoids.

Indexed as

OrganoidsPluripotent Stem CellsAdultAnimalsBiocompatible MaterialsHumansHydrogelsInfant, NewbornLungMiceTissue EngineeringTissue ScaffoldsBiocompatible MaterialsHydrogelsLungOrganoidsScaffoldsStem cells

Identifiers

PMID31951973
PMCPMC6996062
OpenAlexW2997229843

What Socratic holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.