Evidence map›Paper›PMID 32034090›Full record

ArticleThe Journal of biological chemistry2020

Generation and validation of a PITX2-EGFP reporter line of human induced pluripotent stem cells enables isolation of periocular mesenchymal cells.

Toru Okubo, Ryuhei Hayashi, Shun Shibata, Yuji Kudo, Yuki Ishikawa, Saki Inoue, Yuki Kobayashi, Ai Honda, Yoichi Honma, Satoshi Kawasaki and 1 more

Open access · hybridAbstract readValidation Study
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of biological chemistry, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.3field-weighted citation impact, top 44% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 5 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 2 institutions in 1 country.

Toru OkuboDepartment of Stem Cells and Applied Medicine, Osaka University Graduate School of Medicine, Suita, Osaka 565-0871, Japan; Basic Research Development Division, Rohto Pharmaceutical Co., Ltd., Osaka 544-8666, Japan.
Ryuhei HayashiDepartment of Stem Cells and Applied Medicine, Osaka University Graduate School of Medicine, Suita, Osaka 565-0871, Japan; Department of Ophthalmology, Graduate School of Medicine, Osaka University, Suita, Osaka 565-0871, Japan. Electronic address: ryuhei.hayashi@ophthal.med.osaka-u.ac.jp.
Shun ShibataDepartment of Stem Cells and Applied Medicine, Osaka University Graduate School of Medicine, Suita, Osaka 565-0871, Japan; Basic Research Development Division, Rohto Pharmaceutical Co., Ltd., Osaka 544-8666, Japan.
Yuji KudoDepartment of Stem Cells and Applied Medicine, Osaka University Graduate School of Medicine, Suita, Osaka 565-0871, Japan; Basic Research Development Division, Rohto Pharmaceutical Co., Ltd., Osaka 544-8666, Japan.
Yuki IshikawaDepartment of Ophthalmology, Graduate School of Medicine, Osaka University, Suita, Osaka 565-0871, Japan.
Saki InoueDepartment of Ophthalmology, Graduate School of Medicine, Osaka University, Suita, Osaka 565-0871, Japan.
Yuki KobayashiDepartment of Ophthalmology, Graduate School of Medicine, Osaka University, Suita, Osaka 565-0871, Japan.
Ai HondaDepartment of Ophthalmology, Graduate School of Medicine, Osaka University, Suita, Osaka 565-0871, Japan.
Yoichi HonmaDepartment of Stem Cells and Applied Medicine, Osaka University Graduate School of Medicine, Suita, Osaka 565-0871, Japan; Basic Research Development Division, Rohto Pharmaceutical Co., Ltd., Osaka 544-8666, Japan.
Satoshi KawasakiDepartment of Ophthalmology, Graduate School of Medicine, Osaka University, Suita, Osaka 565-0871, Japan.
Kohji NishidaDepartment of Ophthalmology, Graduate School of Medicine, Osaka University, Suita, Osaka 565-0871, Japan; Integrated Frontier Research for Medical Science Division, Institute for Open and Transdisciplinary Research Initiatives, Osaka University, Suita, Osaka 565-0871, Japan.
The University of Osaka · JPRohto Pharmaceutical (Japan) · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

PITX2 (Paired-like homeodomain transcription factor 2) plays important roles in asymmetric development of the internal organs and symmetric development of eye tissues. During eye development, cranial neural crest cells migrate from the neural tube and form the periocular mesenchyme (POM). POM cells differentiate into several ocular cell types, such as corneal endothelial cells, keratocytes, and some ocular mesenchymal cells. In this study, we used transcription activator-like effector nuclease technology to establish a human induced pluripotent stem cell (hiPSC) line expressing a fluorescent reporter gene from the

Indexed as

Cell SeparationGenes, ReporterAnimalsCell LineClone CellsEctodermEmbryo, MammalianEyeFluorescenceGreen Fluorescent ProteinsHomeobox Protein PITX2Homeodomain ProteinsHumansInduced Pluripotent Stem CellsMesenchymal Stem CellsMice, Inbred ICRenhanced green fluorescent proteinGreen Fluorescent ProteinsHomeobox Protein PITX2Homeodomain ProteinsTranscription Factorseye developmentinduced pluripotent stem cell (iPS cell) (iPSC)neural crest cellpaired-like homeodomain transcription factor 2 (PITX2)periocular mesenchyme (POM)regenerative medicinereporter genestem cellstranscription activator–like effector nuclease (TALEN)

Identifiers

PMID32034090
PMCPMC7076207
OpenAlexW3005226072

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.